An electrofusion chip with a cell delivery system driven by surface tension

Jongil Ju, Jung Moon Ko, Hyeon Cheol Cha, Joong Yull Park, Chang Hwan Im, Sang Hoon Lee

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

We have fabricated an electric cell fusion chip with an embedded cell delivery function driven by surface tension and evaluated its performance with several types of plant cells. The chip consists of a polydimethylsiloxane-based microchannel with a fusion chamber and gold-titanium (Au-Ti) electrodes. The velocity profiles of the microfluid in the channel and fusion chamber were calculated to predict cell movement, and the electric field distribution between the electrodes was also calculated in order to determine the appropriate electrode shape. The range of the fluid velocity in the fusion chamber is 20-50 νm s-1 and the measured speed of the cells is approximately 45 νm s-1, which is sufficiently slow for the motion of the cells in the fusion chamber to be monitored and controlled. We measured the variation of the pearl chain ratio with frequency for five kinds of plant cells, and determined that the optimal frequency for pearl chain formation is 1.5 MHz. The electrofusion of cells was successfully carried out under ac field (amplitude: 0.4-0.5 kV cm-1, frequency: 1.5 MHz) and dc pulse (amplitude: 1.0 kV cm-1, duration: 20 ms) conditions.

Original languageEnglish
Article number015004
JournalJournal of Micromechanics and Microengineering
Volume19
Issue number1
DOIs
Publication statusPublished - 2009 Mar 25

Fingerprint

Surface tension
Fusion reactions
Electrodes
Electric batteries
Polydimethylsiloxane
Microchannels
Titanium
Gold
Cells
Electric fields
Fluids
Plant Cells

ASJC Scopus subject areas

  • Mechanical Engineering
  • Electrical and Electronic Engineering
  • Mechanics of Materials
  • Electronic, Optical and Magnetic Materials

Cite this

An electrofusion chip with a cell delivery system driven by surface tension. / Ju, Jongil; Ko, Jung Moon; Cha, Hyeon Cheol; Park, Joong Yull; Im, Chang Hwan; Lee, Sang Hoon.

In: Journal of Micromechanics and Microengineering, Vol. 19, No. 1, 015004, 25.03.2009.

Research output: Contribution to journalArticle

Ju, Jongil ; Ko, Jung Moon ; Cha, Hyeon Cheol ; Park, Joong Yull ; Im, Chang Hwan ; Lee, Sang Hoon. / An electrofusion chip with a cell delivery system driven by surface tension. In: Journal of Micromechanics and Microengineering. 2009 ; Vol. 19, No. 1.
@article{434cfb2208f3470db7eabe8d47ddd8c6,
title = "An electrofusion chip with a cell delivery system driven by surface tension",
abstract = "We have fabricated an electric cell fusion chip with an embedded cell delivery function driven by surface tension and evaluated its performance with several types of plant cells. The chip consists of a polydimethylsiloxane-based microchannel with a fusion chamber and gold-titanium (Au-Ti) electrodes. The velocity profiles of the microfluid in the channel and fusion chamber were calculated to predict cell movement, and the electric field distribution between the electrodes was also calculated in order to determine the appropriate electrode shape. The range of the fluid velocity in the fusion chamber is 20-50 νm s-1 and the measured speed of the cells is approximately 45 νm s-1, which is sufficiently slow for the motion of the cells in the fusion chamber to be monitored and controlled. We measured the variation of the pearl chain ratio with frequency for five kinds of plant cells, and determined that the optimal frequency for pearl chain formation is 1.5 MHz. The electrofusion of cells was successfully carried out under ac field (amplitude: 0.4-0.5 kV cm-1, frequency: 1.5 MHz) and dc pulse (amplitude: 1.0 kV cm-1, duration: 20 ms) conditions.",
author = "Jongil Ju and Ko, {Jung Moon} and Cha, {Hyeon Cheol} and Park, {Joong Yull} and Im, {Chang Hwan} and Lee, {Sang Hoon}",
year = "2009",
month = "3",
day = "25",
doi = "10.1088/0960-1317/19/1/015004",
language = "English",
volume = "19",
journal = "Journal of Micromechanics and Microengineering",
issn = "0960-1317",
publisher = "IOP Publishing Ltd.",
number = "1",

}

TY - JOUR

T1 - An electrofusion chip with a cell delivery system driven by surface tension

AU - Ju, Jongil

AU - Ko, Jung Moon

AU - Cha, Hyeon Cheol

AU - Park, Joong Yull

AU - Im, Chang Hwan

AU - Lee, Sang Hoon

PY - 2009/3/25

Y1 - 2009/3/25

N2 - We have fabricated an electric cell fusion chip with an embedded cell delivery function driven by surface tension and evaluated its performance with several types of plant cells. The chip consists of a polydimethylsiloxane-based microchannel with a fusion chamber and gold-titanium (Au-Ti) electrodes. The velocity profiles of the microfluid in the channel and fusion chamber were calculated to predict cell movement, and the electric field distribution between the electrodes was also calculated in order to determine the appropriate electrode shape. The range of the fluid velocity in the fusion chamber is 20-50 νm s-1 and the measured speed of the cells is approximately 45 νm s-1, which is sufficiently slow for the motion of the cells in the fusion chamber to be monitored and controlled. We measured the variation of the pearl chain ratio with frequency for five kinds of plant cells, and determined that the optimal frequency for pearl chain formation is 1.5 MHz. The electrofusion of cells was successfully carried out under ac field (amplitude: 0.4-0.5 kV cm-1, frequency: 1.5 MHz) and dc pulse (amplitude: 1.0 kV cm-1, duration: 20 ms) conditions.

AB - We have fabricated an electric cell fusion chip with an embedded cell delivery function driven by surface tension and evaluated its performance with several types of plant cells. The chip consists of a polydimethylsiloxane-based microchannel with a fusion chamber and gold-titanium (Au-Ti) electrodes. The velocity profiles of the microfluid in the channel and fusion chamber were calculated to predict cell movement, and the electric field distribution between the electrodes was also calculated in order to determine the appropriate electrode shape. The range of the fluid velocity in the fusion chamber is 20-50 νm s-1 and the measured speed of the cells is approximately 45 νm s-1, which is sufficiently slow for the motion of the cells in the fusion chamber to be monitored and controlled. We measured the variation of the pearl chain ratio with frequency for five kinds of plant cells, and determined that the optimal frequency for pearl chain formation is 1.5 MHz. The electrofusion of cells was successfully carried out under ac field (amplitude: 0.4-0.5 kV cm-1, frequency: 1.5 MHz) and dc pulse (amplitude: 1.0 kV cm-1, duration: 20 ms) conditions.

UR - http://www.scopus.com/inward/record.url?scp=62649163684&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=62649163684&partnerID=8YFLogxK

U2 - 10.1088/0960-1317/19/1/015004

DO - 10.1088/0960-1317/19/1/015004

M3 - Article

VL - 19

JO - Journal of Micromechanics and Microengineering

JF - Journal of Micromechanics and Microengineering

SN - 0960-1317

IS - 1

M1 - 015004

ER -