An endogenous acid-sensitive K+ channel expressed in COS-7 cells

Dawon Kang, Jun Ho La, Eun Jin Kim, Jae Yong Park, Seong Geun Hong, Jaehee Han

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

COS-7 cells originally isolated from monkey kidney and used in many transfection studies were found to express a background K+ channel and therefore, its biophysical and pharmacological properties were examined. In cell-attached patches, a 32-pS K+ channel with a linear current-voltage relationship could be recorded. The open probability was highly voltage-dependent, with greater channel activity at depolarized potentials. The channel was markedly sensitive to changes in extracellular pH (pHo), showing a 70 ± 10% inhibition by changing the pHo from 7.3 to 6.3. Arachidonic acid (5 μM) augmented channel activity 12-fold. Applying negative pressure (-40 mmHg) to the membrane patch also increased channel activity by 4-fold. These results show that COS-7 cells express a K+ channel with unique properties that must be considered when using these cells as transfection system.

Original languageEnglish
Pages (from-to)1231-1236
Number of pages6
JournalBiochemical and biophysical research communications
Volume341
Issue number4
DOIs
Publication statusPublished - 2006 Mar 24

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Keywords

  • Arachidonic acid
  • Background
  • COS-7 cells
  • Hydrogen
  • Potassium channel
  • Pressure

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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