An Escherichia coli biosensor capable of detecting both genotoxic and oxidative damage

R. J. Mitchell, Man Bock Gu

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

A two-plasmid dual reporter Escherichia coli biosensor was developed using the genes for bacterial bioluminescence and a mutant of the green fluorescent protein, GFPuv4. To achieve this, the two plasmids, which were derivatives of pBR322 and pACYC184, had compatible origins of replication and different antibiotic selection markers: ampicillin and tetracycline. The parent strains DK1 and ACRG43, each carrying a single plasmid with one of the fusion genes (strain DK1 harboring a fusion of the katG promoter to the lux operon while in ACRG43, the recA promoter was fused with the GFP gene), were responsive to oxidative and DNA damage, respectively, resulting in higher bioluminescence or fluorescence under the relevant toxic conditions. The responses of the dual sensor strain, DUAL22, to various toxicants, e.g., mitomycin C, N-methyl-N-nitro-N-nitrosoguanidine, hydrogen peroxide and cadmium chloride, were characterized and compared with the responses of the parent strains to the same chemicals. Finally, several chemical mixtures that cause various stress responses were tested to demonstrate the ability of this biosensor to detect specific stress responses within a multiple toxicity environment.

Original languageEnglish
Pages (from-to)46-52
Number of pages7
JournalApplied Microbiology and Biotechnology
Volume64
Issue number1
DOIs
Publication statusPublished - 2004 Mar 1
Externally publishedYes

Fingerprint

Biosensing Techniques
Biosensors
Escherichia coli
Bioluminescence
Plasmids
Genes
Fusion reactions
Cadmium chloride
Bacterial Genes
Methylnitronitrosoguanidine
Cadmium Chloride
Replication Origin
Hydrochloric Acid
Poisons
Gene Fusion
Mitomycin
Antibiotics
Ampicillin
Operon
Green Fluorescent Proteins

ASJC Scopus subject areas

  • Biotechnology
  • Microbiology
  • Bioengineering

Cite this

An Escherichia coli biosensor capable of detecting both genotoxic and oxidative damage. / Mitchell, R. J.; Gu, Man Bock.

In: Applied Microbiology and Biotechnology, Vol. 64, No. 1, 01.03.2004, p. 46-52.

Research output: Contribution to journalArticle

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