An evaluation of the neonatal immune system using a Listeria infection model

Hyun Jung Byun, Woon Won Jung, Jong Bae Lee, Hee Yong Chung, Donggeun Sul, Sang Joon Kim, Chung Gyu Park, Inho Choi, Kwang Woo Hwang, Taehoon Chun

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13 Citations (Scopus)

Abstract

Background: T helper 1 (Th1)/T helper 2 (Th2)-biased cytokine regulation may be another reason that neonates are much more susceptible to infectious disease than are adults. Objectives: We attempted to determine the ability of neonatal mice to direct the Th1 phenotype against Listeria monocytogenes (LM), because LM, an intracellular Gram-positive bacterium, induces profound cellular immunity by Th1 cells in vivo. Methods: In order to determine whether neonatal mice evidence strong Th1 activity during LM infection, neonatal mice were compared with adult mice with regard to susceptibility to LM, cytotoxic T lymphocyte activity, and cytokine profiles. Neonatal gene profiles relevant to Th1 and Th2 differentiation during LM infection were also compared between neonatal and adult mice, via real-time PCR and RT-PCR. Results: Neonatal mice were found to be far more susceptible to LM infection than adult mice, due to a lack in the induction of cytotoxic T cell activity, coupled with poor IFN-γ secretion. Further, LM-infected neonatal mice evidenced much lower levels of expression of Th1-type immune components, including IL-12, IFN-γ, Delta-4 and T-bet, as compared to those features in adult mice. These results may be due to the comparably lower expressions of mannose-bind lectins and some of toll-like receptors (TLRs) such as TLR-5, -6 and -9, necessary mediators to develop Th1 immune responses. Conclusions: Neonatal mice may not mount an adequate Th1 type immune response due to a significantly lower expression of Th1-type immune components as compared to adult mice, even when forced into a Th1-prone environment.

Original languageEnglish
Pages (from-to)83-90
Number of pages8
JournalNeonatology
Volume92
Issue number2
DOIs
Publication statusPublished - 2007 Aug

Keywords

  • Listeria infection
  • Neonate
  • T helper 1 and T helper 2 imbalance

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Developmental Biology

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    Byun, H. J., Jung, W. W., Lee, J. B., Chung, H. Y., Sul, D., Kim, S. J., Park, C. G., Choi, I., Hwang, K. W., & Chun, T. (2007). An evaluation of the neonatal immune system using a Listeria infection model. Neonatology, 92(2), 83-90. https://doi.org/10.1159/000100806