An evaluation of the neonatal immune system using a Listeria infection model

Hyun Jung Byun, Woon Won Jung, Jong Bae Lee, Hee Yong Chung, Dong Geun Sul, Sang Joon Kim, Chung Gyu Park, Inho Choi, Kwang Woo Hwang, Taehoon Chun

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Background: T helper 1 (Th1)/T helper 2 (Th2)-biased cytokine regulation may be another reason that neonates are much more susceptible to infectious disease than are adults. Objectives: We attempted to determine the ability of neonatal mice to direct the Th1 phenotype against Listeria monocytogenes (LM), because LM, an intracellular Gram-positive bacterium, induces profound cellular immunity by Th1 cells in vivo. Methods: In order to determine whether neonatal mice evidence strong Th1 activity during LM infection, neonatal mice were compared with adult mice with regard to susceptibility to LM, cytotoxic T lymphocyte activity, and cytokine profiles. Neonatal gene profiles relevant to Th1 and Th2 differentiation during LM infection were also compared between neonatal and adult mice, via real-time PCR and RT-PCR. Results: Neonatal mice were found to be far more susceptible to LM infection than adult mice, due to a lack in the induction of cytotoxic T cell activity, coupled with poor IFN-γ secretion. Further, LM-infected neonatal mice evidenced much lower levels of expression of Th1-type immune components, including IL-12, IFN-γ, Delta-4 and T-bet, as compared to those features in adult mice. These results may be due to the comparably lower expressions of mannose-bind lectins and some of toll-like receptors (TLRs) such as TLR-5, -6 and -9, necessary mediators to develop Th1 immune responses. Conclusions: Neonatal mice may not mount an adequate Th1 type immune response due to a significantly lower expression of Th1-type immune components as compared to adult mice, even when forced into a Th1-prone environment.

Original languageEnglish
Pages (from-to)83-90
Number of pages8
JournalNeonatology
Volume92
Issue number2
DOIs
Publication statusPublished - 2007 Aug 1

Fingerprint

Listeriosis
Immune System
Listeria monocytogenes
Toll-Like Receptor 6
Toll-Like Receptor 5
Cytokines
Th1 Cells
Toll-Like Receptors
Gram-Positive Bacteria
Cytotoxic T-Lymphocytes
Interleukin-12
Mannose
Lectins
Cellular Immunity
Communicable Diseases
Real-Time Polymerase Chain Reaction

Keywords

  • Listeria infection
  • Neonate
  • T helper 1 and T helper 2 imbalance

ASJC Scopus subject areas

  • Developmental Biology
  • Pediatrics, Perinatology, and Child Health

Cite this

An evaluation of the neonatal immune system using a Listeria infection model. / Byun, Hyun Jung; Jung, Woon Won; Lee, Jong Bae; Chung, Hee Yong; Sul, Dong Geun; Kim, Sang Joon; Park, Chung Gyu; Choi, Inho; Hwang, Kwang Woo; Chun, Taehoon.

In: Neonatology, Vol. 92, No. 2, 01.08.2007, p. 83-90.

Research output: Contribution to journalArticle

Byun, HJ, Jung, WW, Lee, JB, Chung, HY, Sul, DG, Kim, SJ, Park, CG, Choi, I, Hwang, KW & Chun, T 2007, 'An evaluation of the neonatal immune system using a Listeria infection model', Neonatology, vol. 92, no. 2, pp. 83-90. https://doi.org/10.1159/000100806
Byun, Hyun Jung ; Jung, Woon Won ; Lee, Jong Bae ; Chung, Hee Yong ; Sul, Dong Geun ; Kim, Sang Joon ; Park, Chung Gyu ; Choi, Inho ; Hwang, Kwang Woo ; Chun, Taehoon. / An evaluation of the neonatal immune system using a Listeria infection model. In: Neonatology. 2007 ; Vol. 92, No. 2. pp. 83-90.
@article{ef21fe3e1d434a759ccb9fb491f24d35,
title = "An evaluation of the neonatal immune system using a Listeria infection model",
abstract = "Background: T helper 1 (Th1)/T helper 2 (Th2)-biased cytokine regulation may be another reason that neonates are much more susceptible to infectious disease than are adults. Objectives: We attempted to determine the ability of neonatal mice to direct the Th1 phenotype against Listeria monocytogenes (LM), because LM, an intracellular Gram-positive bacterium, induces profound cellular immunity by Th1 cells in vivo. Methods: In order to determine whether neonatal mice evidence strong Th1 activity during LM infection, neonatal mice were compared with adult mice with regard to susceptibility to LM, cytotoxic T lymphocyte activity, and cytokine profiles. Neonatal gene profiles relevant to Th1 and Th2 differentiation during LM infection were also compared between neonatal and adult mice, via real-time PCR and RT-PCR. Results: Neonatal mice were found to be far more susceptible to LM infection than adult mice, due to a lack in the induction of cytotoxic T cell activity, coupled with poor IFN-γ secretion. Further, LM-infected neonatal mice evidenced much lower levels of expression of Th1-type immune components, including IL-12, IFN-γ, Delta-4 and T-bet, as compared to those features in adult mice. These results may be due to the comparably lower expressions of mannose-bind lectins and some of toll-like receptors (TLRs) such as TLR-5, -6 and -9, necessary mediators to develop Th1 immune responses. Conclusions: Neonatal mice may not mount an adequate Th1 type immune response due to a significantly lower expression of Th1-type immune components as compared to adult mice, even when forced into a Th1-prone environment.",
keywords = "Listeria infection, Neonate, T helper 1 and T helper 2 imbalance",
author = "Byun, {Hyun Jung} and Jung, {Woon Won} and Lee, {Jong Bae} and Chung, {Hee Yong} and Sul, {Dong Geun} and Kim, {Sang Joon} and Park, {Chung Gyu} and Inho Choi and Hwang, {Kwang Woo} and Taehoon Chun",
year = "2007",
month = "8",
day = "1",
doi = "10.1159/000100806",
language = "English",
volume = "92",
pages = "83--90",
journal = "Neonatology",
issn = "1661-7800",
publisher = "S. Karger AG",
number = "2",

}

TY - JOUR

T1 - An evaluation of the neonatal immune system using a Listeria infection model

AU - Byun, Hyun Jung

AU - Jung, Woon Won

AU - Lee, Jong Bae

AU - Chung, Hee Yong

AU - Sul, Dong Geun

AU - Kim, Sang Joon

AU - Park, Chung Gyu

AU - Choi, Inho

AU - Hwang, Kwang Woo

AU - Chun, Taehoon

PY - 2007/8/1

Y1 - 2007/8/1

N2 - Background: T helper 1 (Th1)/T helper 2 (Th2)-biased cytokine regulation may be another reason that neonates are much more susceptible to infectious disease than are adults. Objectives: We attempted to determine the ability of neonatal mice to direct the Th1 phenotype against Listeria monocytogenes (LM), because LM, an intracellular Gram-positive bacterium, induces profound cellular immunity by Th1 cells in vivo. Methods: In order to determine whether neonatal mice evidence strong Th1 activity during LM infection, neonatal mice were compared with adult mice with regard to susceptibility to LM, cytotoxic T lymphocyte activity, and cytokine profiles. Neonatal gene profiles relevant to Th1 and Th2 differentiation during LM infection were also compared between neonatal and adult mice, via real-time PCR and RT-PCR. Results: Neonatal mice were found to be far more susceptible to LM infection than adult mice, due to a lack in the induction of cytotoxic T cell activity, coupled with poor IFN-γ secretion. Further, LM-infected neonatal mice evidenced much lower levels of expression of Th1-type immune components, including IL-12, IFN-γ, Delta-4 and T-bet, as compared to those features in adult mice. These results may be due to the comparably lower expressions of mannose-bind lectins and some of toll-like receptors (TLRs) such as TLR-5, -6 and -9, necessary mediators to develop Th1 immune responses. Conclusions: Neonatal mice may not mount an adequate Th1 type immune response due to a significantly lower expression of Th1-type immune components as compared to adult mice, even when forced into a Th1-prone environment.

AB - Background: T helper 1 (Th1)/T helper 2 (Th2)-biased cytokine regulation may be another reason that neonates are much more susceptible to infectious disease than are adults. Objectives: We attempted to determine the ability of neonatal mice to direct the Th1 phenotype against Listeria monocytogenes (LM), because LM, an intracellular Gram-positive bacterium, induces profound cellular immunity by Th1 cells in vivo. Methods: In order to determine whether neonatal mice evidence strong Th1 activity during LM infection, neonatal mice were compared with adult mice with regard to susceptibility to LM, cytotoxic T lymphocyte activity, and cytokine profiles. Neonatal gene profiles relevant to Th1 and Th2 differentiation during LM infection were also compared between neonatal and adult mice, via real-time PCR and RT-PCR. Results: Neonatal mice were found to be far more susceptible to LM infection than adult mice, due to a lack in the induction of cytotoxic T cell activity, coupled with poor IFN-γ secretion. Further, LM-infected neonatal mice evidenced much lower levels of expression of Th1-type immune components, including IL-12, IFN-γ, Delta-4 and T-bet, as compared to those features in adult mice. These results may be due to the comparably lower expressions of mannose-bind lectins and some of toll-like receptors (TLRs) such as TLR-5, -6 and -9, necessary mediators to develop Th1 immune responses. Conclusions: Neonatal mice may not mount an adequate Th1 type immune response due to a significantly lower expression of Th1-type immune components as compared to adult mice, even when forced into a Th1-prone environment.

KW - Listeria infection

KW - Neonate

KW - T helper 1 and T helper 2 imbalance

UR - http://www.scopus.com/inward/record.url?scp=34547788269&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34547788269&partnerID=8YFLogxK

U2 - 10.1159/000100806

DO - 10.1159/000100806

M3 - Article

VL - 92

SP - 83

EP - 90

JO - Neonatology

JF - Neonatology

SN - 1661-7800

IS - 2

ER -