In the case of the railway bridges, uplift forces were occurred at the edge of the segments when vehicular loa ds were applied. These forces made the compressive and tensile forces occur in the fastening system. There fore, the structural analysis was performed to investigate the safety of fastening system which was modeled as one directional spring element. In this case, the stiffness of the spring element was obtained from experimental study which was conducted by compressive load. For that reason, to perform rational and exact structural analysis, the translational stiffness of the fastening system obtained from the experimental study applied the tensile load and the rotational stiffness should be considered because it was occurred the tensile force as well as the compressive force in fastening system. In this study, an elastic and inelastic experimental study was performed for six specimens. The translational stiffness along the vertical axis of rail and the rotational stiffness along the strong axis of rail were investigated. Also structural behavior of the fastening system was analyzed.