An Extremely Alkaline Novel Xylanase from a Newly Isolated Streptomyces Strain Cultivated in Corncob Medium

Jaya Ram Simkhada, Hah Young Yoo, Yun Hee Choi, Seung Wook Kim, Jin Cheol Yoo

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Streptomyces sp. CS802, recently isolated from Korean soil, produced xylanase in corncob medium. An extracellular xylanase (Xyn802) was purified by a single-step gel filtration and biochemical properties were studied. It showed high activity in extremely alkaline condition with optimum pH at 12.0 and exhibited stability between pH 7.5 and 13.0. It produced xylobiose and xylotriose as the major products from xylan, suggesting its endoxylanase nature. N-terminal amino acid sequences of Xyn802 were ADRNANRD which are significantly different from the reported xylanase. The activity was enhanced by various detergents and a reducing agent and stable in various organic solvents. Xyn802 produced by utilizing corncob, an agro-waste material, might be a novel xylanase based on its peculiar biochemical characteristics, and it can be a suitable candidate for the production of xylooligosaccharides including other useful products.

Original languageEnglish
Pages (from-to)2017-2027
Number of pages11
JournalApplied Biochemistry and Biotechnology
Volume168
Issue number7
DOIs
Publication statusPublished - 2012 Dec 1

Fingerprint

Endo-1,4-beta Xylanases
Xylans
Reducing Agents
Detergents
Streptomyces
Reducing agents
Organic solvents
Amino acids
Gels
Soils
Amino Acids
Gel Chromatography
Amino Acid Sequence
Soil
xylotriose
xylobiose
xylooligosaccharide

Keywords

  • Agro-waste
  • Corncob
  • Hyperalkaline xylanase
  • Streptomyces
  • Xylooligosaccharides production

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Molecular Biology

Cite this

An Extremely Alkaline Novel Xylanase from a Newly Isolated Streptomyces Strain Cultivated in Corncob Medium. / Simkhada, Jaya Ram; Yoo, Hah Young; Choi, Yun Hee; Kim, Seung Wook; Yoo, Jin Cheol.

In: Applied Biochemistry and Biotechnology, Vol. 168, No. 7, 01.12.2012, p. 2017-2027.

Research output: Contribution to journalArticle

Simkhada, Jaya Ram ; Yoo, Hah Young ; Choi, Yun Hee ; Kim, Seung Wook ; Yoo, Jin Cheol. / An Extremely Alkaline Novel Xylanase from a Newly Isolated Streptomyces Strain Cultivated in Corncob Medium. In: Applied Biochemistry and Biotechnology. 2012 ; Vol. 168, No. 7. pp. 2017-2027.
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