An immunocytochemical study of mitochondrial manganese-superoxide dismutase in the rat hippocampus after kainate administration

Hyoung Chun Kim, Wang Kee Jhoo, Won Ki Kim, Jeong Hye Suh, Eun Joo Shin, Kanefusa Kato, Kwang Ho Ko

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

We examined the immunocytochemical distribution of mitochondrial Mn- superoxide dismutase (SOD-2) in the rat hippocampus after systemic injection of kainic acid (KA), in order to understand SOD-2-responsible antioxidant defense mechanism during the neurodegenerative process. SOD-2 immunostaining was more intense in CA3 pyramidal neurons than in CA1 neurons in the normal hippocampus. The immunoreactivity in region CA1 was reduced without significant neuronal losses within 12 h of KA injection. The CA1 and CA3 neurons lost their immunoreactivity, whereas SOD-2-positive glia-like cells proliferated, mainly throughout the CA1 sector, and had intense immunoreactivity 3 and 7 days after KA injection. This immunocytochemical distribution of SOD-2-positive non-neuronal elements was similar to that of glial fibrillary acidic protein (GFAP) and S-100 protein-positive cells. Activated microglial cells selectively marked with lectin occurred in the areas affected by the KA-induced lesion. Double-labeling immunocytochemistry showed the co-localization of SOD-2-positive non-neuronal cells and GFAP or S-100 protein-like immunoreactivity in the same cells. This suggests that astroglial cells mobilized to synthesize of SOD-2 protein in a response to KA toxicity designed to reduce the oxidative damage. (C) 2000 Elsevier Science Ireland Ltd.

Original languageEnglish
Pages (from-to)65-68
Number of pages4
JournalNeuroscience Letters
Volume281
Issue number1
DOIs
Publication statusPublished - 2000 Mar 3

Keywords

  • Astrocytes
  • Free radicals
  • Hippocampus
  • Kainic acid
  • Microglia
  • Mitochondrial Mn-superoxide dismutase
  • Neurodegeneration

ASJC Scopus subject areas

  • Neuroscience(all)

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