An immunocytochemical study of mitochondrial manganese-superoxide dismutase in the rat hippocampus after kainate administration

Hyoung Chun Kim, Wang K. Jhoo, Won-Ki Kim, Jeong H. Suh, Eun J. Shin, Kanefusa Kato, Kwang Ho Ko

Research output: Contribution to journalArticle

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Abstract

We examined the immunocytochemical distribution of mitochondrial Mn- superoxide dismutase (SOD-2) in the rat hippocampus after systemic injection of kainic acid (KA), in order to understand SOD-2-responsible antioxidant defense mechanism during the neurodegenerative process. SOD-2 immunostaining was more intense in CA3 pyramidal neurons than in CA1 neurons in the normal hippocampus. The immunoreactivity in region CA1 was reduced without significant neuronal losses within 12 h of KA injection. The CA1 and CA3 neurons lost their immunoreactivity, whereas SOD-2-positive glia-like cells proliferated, mainly throughout the CA1 sector, and had intense immunoreactivity 3 and 7 days after KA injection. This immunocytochemical distribution of SOD-2-positive non-neuronal elements was similar to that of glial fibrillary acidic protein (GFAP) and S-100 protein-positive cells. Activated microglial cells selectively marked with lectin occurred in the areas affected by the KA-induced lesion. Double-labeling immunocytochemistry showed the co-localization of SOD-2-positive non-neuronal cells and GFAP or S-100 protein-like immunoreactivity in the same cells. This suggests that astroglial cells mobilized to synthesize of SOD-2 protein in a response to KA toxicity designed to reduce the oxidative damage. (C) 2000 Elsevier Science Ireland Ltd.

Original languageEnglish
Pages (from-to)65-68
Number of pages4
JournalNeuroscience Letters
Volume281
Issue number1
DOIs
Publication statusPublished - 2000 Mar 3
Externally publishedYes

Fingerprint

Kainic Acid
Superoxide Dismutase
Hippocampus
S100 Proteins
Glial Fibrillary Acidic Protein
Protein S
Injections
Neurons
Pyramidal Cells
Lectins
Neuroglia
Antioxidants
Immunohistochemistry
Proteins

Keywords

  • Astrocytes
  • Free radicals
  • Hippocampus
  • Kainic acid
  • Microglia
  • Mitochondrial Mn-superoxide dismutase
  • Neurodegeneration

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

An immunocytochemical study of mitochondrial manganese-superoxide dismutase in the rat hippocampus after kainate administration. / Kim, Hyoung Chun; Jhoo, Wang K.; Kim, Won-Ki; Suh, Jeong H.; Shin, Eun J.; Kato, Kanefusa; Ho Ko, Kwang.

In: Neuroscience Letters, Vol. 281, No. 1, 03.03.2000, p. 65-68.

Research output: Contribution to journalArticle

Kim, Hyoung Chun ; Jhoo, Wang K. ; Kim, Won-Ki ; Suh, Jeong H. ; Shin, Eun J. ; Kato, Kanefusa ; Ho Ko, Kwang. / An immunocytochemical study of mitochondrial manganese-superoxide dismutase in the rat hippocampus after kainate administration. In: Neuroscience Letters. 2000 ; Vol. 281, No. 1. pp. 65-68.
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