An improved procedure for the recovery of pancreatic enzymes with enhanced activity

Moo Heon Kang, Jeewon Lee

Research output: Contribution to journalArticle

Abstract

Pancreatic enzymes were recovered with enhanced activity by a newly designed extraction process. The protease activity was increased to the extent of 65x by using MES buffer, 64mM and duodenum enzyme extracts containing enterokinase at autolysis stage. The decrease in amylase activity due presumably to proteolytic degradation was effectively prevented by treating the enzyme solution with calcium prior to the autolysis.

Original languageEnglish
Pages (from-to)419-424
Number of pages6
JournalBiotechnology Techniques
Volume10
Issue number6
Publication statusPublished - 1996
Externally publishedYes

Fingerprint

Autolysis
Recovery
Enzymes
Enteropeptidase
Amylases
Duodenum
Buffers
Peptide Hydrolases
Calcium
Degradation

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Biochemistry

Cite this

An improved procedure for the recovery of pancreatic enzymes with enhanced activity. / Kang, Moo Heon; Lee, Jeewon.

In: Biotechnology Techniques, Vol. 10, No. 6, 1996, p. 419-424.

Research output: Contribution to journalArticle

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