Pancreatic enzymes were recovered with enhanced activity by a newly designed extraction process. The protease activity was increased to the extent of 65x by using MES buffer, 64mM and duodenum enzyme extracts containing enterokinase at autolysis stage. The decrease in amylase activity due presumably to proteolytic degradation was effectively prevented by treating the enzyme solution with calcium prior to the autolysis.
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology