An in vitro evaluation of the antibacterial properties of three mineral trioxide aggregate (MTA) against five oral bacteria

Ryan Jin Young Kim, Myung Ook Kim, Ki Sun Lee, Dong-Yul Lee, Joo Hee Shin

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Objective: The purpose of this study was to evaluate the antibacterial ability of three MTA (MTA-Angelus, Endocem MTA, and ProRoot MTA) against five typical oral bacteria (Streptococcus mutans, Enterococcus faecalis, Lactobacillus rhamnosus, Lactobacillus paracasei, and Porphyromonas gingivalis). Design: For disc diffusion test, each test material was placed into agar plates after inoculation of each bacterial strain. The zones of inhibition of bacterial growth were then measured. Antibacterial broth test was performed by adding the test material into the media. Colony-forming units were counted after incubation with bacteria. The data were analyzed using ANOVA and the Tukey's test. Results: Disc diffusion test showed that the antibacterial activity against S. mutans, L. rhamnosus, L. paracasei, and P. gingivalis ranked in decreasing order of MTA-Angelus > ProRoot MTA > Endocem MTA (p < 0.05). An inhibitory effect against E. faecalis was only observed in Endocem MTA. Antibacterial broth test showed that the antibacterial activity against all bacteria was Endocem MTA > MTA-Angelus > ProRoot MTA (p < 0.05). Conclusion: Discrepant results were obtained from the disc diffusion and antibacterial broth test, with MTA-Angelus and Endocem MTA being most effective, respectively. Both tests revealed that the most resistant bacteria was E. faecalis, which was not susceptible at all, except to Endocem MTA in disc diffusion test.

Original languageEnglish
Pages (from-to)1497-1502
Number of pages6
JournalArchives of Oral Biology
Volume60
Issue number10
DOIs
Publication statusPublished - 2015 Aug 11

Fingerprint

Bacteria
Lactobacillus rhamnosus
Porphyromonas gingivalis
Streptococcus mutans
Enterococcus faecalis
In Vitro Techniques
mineral trioxide aggregate
Agar
Analysis of Variance
Stem Cells
endocem
Growth

Keywords

  • Base
  • Disc diffusion test
  • Endodontics
  • MTA
  • Pulp capping
  • Zone of inhibition

ASJC Scopus subject areas

  • Otorhinolaryngology
  • Dentistry(all)
  • Cell Biology

Cite this

An in vitro evaluation of the antibacterial properties of three mineral trioxide aggregate (MTA) against five oral bacteria. / Kim, Ryan Jin Young; Kim, Myung Ook; Lee, Ki Sun; Lee, Dong-Yul; Shin, Joo Hee.

In: Archives of Oral Biology, Vol. 60, No. 10, 11.08.2015, p. 1497-1502.

Research output: Contribution to journalArticle

Kim, Ryan Jin Young ; Kim, Myung Ook ; Lee, Ki Sun ; Lee, Dong-Yul ; Shin, Joo Hee. / An in vitro evaluation of the antibacterial properties of three mineral trioxide aggregate (MTA) against five oral bacteria. In: Archives of Oral Biology. 2015 ; Vol. 60, No. 10. pp. 1497-1502.
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