Abstract
Objective: The purpose of this study was to evaluate the antibacterial ability of three MTA (MTA-Angelus, Endocem MTA, and ProRoot MTA) against five typical oral bacteria (Streptococcus mutans, Enterococcus faecalis, Lactobacillus rhamnosus, Lactobacillus paracasei, and Porphyromonas gingivalis). Design: For disc diffusion test, each test material was placed into agar plates after inoculation of each bacterial strain. The zones of inhibition of bacterial growth were then measured. Antibacterial broth test was performed by adding the test material into the media. Colony-forming units were counted after incubation with bacteria. The data were analyzed using ANOVA and the Tukey's test. Results: Disc diffusion test showed that the antibacterial activity against S. mutans, L. rhamnosus, L. paracasei, and P. gingivalis ranked in decreasing order of MTA-Angelus > ProRoot MTA > Endocem MTA (p < 0.05). An inhibitory effect against E. faecalis was only observed in Endocem MTA. Antibacterial broth test showed that the antibacterial activity against all bacteria was Endocem MTA > MTA-Angelus > ProRoot MTA (p < 0.05). Conclusion: Discrepant results were obtained from the disc diffusion and antibacterial broth test, with MTA-Angelus and Endocem MTA being most effective, respectively. Both tests revealed that the most resistant bacteria was E. faecalis, which was not susceptible at all, except to Endocem MTA in disc diffusion test.
| Original language | English |
|---|---|
| Pages (from-to) | 1497-1502 |
| Number of pages | 6 |
| Journal | Archives of Oral Biology |
| Volume | 60 |
| Issue number | 10 |
| DOIs | |
| Publication status | Published - 2015 Aug 11 |
Fingerprint
Keywords
- Base
- Disc diffusion test
- Endodontics
- MTA
- Pulp capping
- Zone of inhibition
ASJC Scopus subject areas
- Otorhinolaryngology
- Dentistry(all)
- Cell Biology
Cite this
An in vitro evaluation of the antibacterial properties of three mineral trioxide aggregate (MTA) against five oral bacteria. / Kim, Ryan Jin Young; Kim, Myung Ook; Lee, Ki Sun; Lee, Dong-Yul; Shin, Joo Hee.
In: Archives of Oral Biology, Vol. 60, No. 10, 11.08.2015, p. 1497-1502.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - An in vitro evaluation of the antibacterial properties of three mineral trioxide aggregate (MTA) against five oral bacteria
AU - Kim, Ryan Jin Young
AU - Kim, Myung Ook
AU - Lee, Ki Sun
AU - Lee, Dong-Yul
AU - Shin, Joo Hee
PY - 2015/8/11
Y1 - 2015/8/11
N2 - Objective: The purpose of this study was to evaluate the antibacterial ability of three MTA (MTA-Angelus, Endocem MTA, and ProRoot MTA) against five typical oral bacteria (Streptococcus mutans, Enterococcus faecalis, Lactobacillus rhamnosus, Lactobacillus paracasei, and Porphyromonas gingivalis). Design: For disc diffusion test, each test material was placed into agar plates after inoculation of each bacterial strain. The zones of inhibition of bacterial growth were then measured. Antibacterial broth test was performed by adding the test material into the media. Colony-forming units were counted after incubation with bacteria. The data were analyzed using ANOVA and the Tukey's test. Results: Disc diffusion test showed that the antibacterial activity against S. mutans, L. rhamnosus, L. paracasei, and P. gingivalis ranked in decreasing order of MTA-Angelus > ProRoot MTA > Endocem MTA (p < 0.05). An inhibitory effect against E. faecalis was only observed in Endocem MTA. Antibacterial broth test showed that the antibacterial activity against all bacteria was Endocem MTA > MTA-Angelus > ProRoot MTA (p < 0.05). Conclusion: Discrepant results were obtained from the disc diffusion and antibacterial broth test, with MTA-Angelus and Endocem MTA being most effective, respectively. Both tests revealed that the most resistant bacteria was E. faecalis, which was not susceptible at all, except to Endocem MTA in disc diffusion test.
AB - Objective: The purpose of this study was to evaluate the antibacterial ability of three MTA (MTA-Angelus, Endocem MTA, and ProRoot MTA) against five typical oral bacteria (Streptococcus mutans, Enterococcus faecalis, Lactobacillus rhamnosus, Lactobacillus paracasei, and Porphyromonas gingivalis). Design: For disc diffusion test, each test material was placed into agar plates after inoculation of each bacterial strain. The zones of inhibition of bacterial growth were then measured. Antibacterial broth test was performed by adding the test material into the media. Colony-forming units were counted after incubation with bacteria. The data were analyzed using ANOVA and the Tukey's test. Results: Disc diffusion test showed that the antibacterial activity against S. mutans, L. rhamnosus, L. paracasei, and P. gingivalis ranked in decreasing order of MTA-Angelus > ProRoot MTA > Endocem MTA (p < 0.05). An inhibitory effect against E. faecalis was only observed in Endocem MTA. Antibacterial broth test showed that the antibacterial activity against all bacteria was Endocem MTA > MTA-Angelus > ProRoot MTA (p < 0.05). Conclusion: Discrepant results were obtained from the disc diffusion and antibacterial broth test, with MTA-Angelus and Endocem MTA being most effective, respectively. Both tests revealed that the most resistant bacteria was E. faecalis, which was not susceptible at all, except to Endocem MTA in disc diffusion test.
KW - Base
KW - Disc diffusion test
KW - Endodontics
KW - MTA
KW - Pulp capping
KW - Zone of inhibition
UR - http://www.scopus.com/inward/record.url?scp=84938831299&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84938831299&partnerID=8YFLogxK
U2 - 10.1016/j.archoralbio.2015.07.014
DO - 10.1016/j.archoralbio.2015.07.014
M3 - Article
C2 - 26263538
AN - SCOPUS:84938831299
VL - 60
SP - 1497
EP - 1502
JO - Archives of Oral Biology
JF - Archives of Oral Biology
SN - 0003-9969
IS - 10
ER -