An innovative sample-to-answer polymer lab-on-a-chip with on-chip reservoirs for the POCT of thyroid stimulating hormone (TSH)

Wooseok Jung, Jungyoup Han, Junhai Kai, Ji Youn Lim, Dong Geun Sul, Chong H. Ahn

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

A new sample-to-answer polymer lab-on-a-chip, which can perform immunoassay with minimum user intervention through on-chip reservoirs for reagents and single-channel assay system, has been designed, developed and successfully characterized as a point-of-care testing (POCT) cartridge for the detection of thyroid stimulating hormone (TSH). Test results were obtained within 30 minutes after a sample was dropped into the POCT cartridge. The analyzed results of TSH showed a linear range of up to 55 μIU mL-1 with the limit of detection (LOD) of 1.9 μIU mL-1 at the signal-to-noise ratio (SNR) of 3. The reagents stored in the on-chip reservoirs maintained more than 97% of their initial volume for 120 days of storage time while the detection antibody retained its activity above 98% for 120 days. The sample-to-answer polymer lab-on-a-chip developed in this work using the mass-producible and low-cost polymer is well suited for the point-of-care testing of rapid in vitro diagnostics (IVD) of TSH.

Original languageEnglish
Pages (from-to)4653-4662
Number of pages10
JournalLab on a Chip - Miniaturisation for Chemistry and Biology
Volume13
Issue number23
DOIs
Publication statusPublished - 2013 Dec 7

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Lab-on-a-chip
Thyrotropin
Polymers
Testing
Signal-To-Noise Ratio
Immunoassay
Antibodies
Limit of Detection
Assays
Signal to noise ratio
Costs and Cost Analysis
Thyroid Hormones
Point-of-Care Testing
Costs

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)
  • Bioengineering
  • Biomedical Engineering

Cite this

An innovative sample-to-answer polymer lab-on-a-chip with on-chip reservoirs for the POCT of thyroid stimulating hormone (TSH). / Jung, Wooseok; Han, Jungyoup; Kai, Junhai; Lim, Ji Youn; Sul, Dong Geun; Ahn, Chong H.

In: Lab on a Chip - Miniaturisation for Chemistry and Biology, Vol. 13, No. 23, 07.12.2013, p. 4653-4662.

Research output: Contribution to journalArticle

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