An insight into the mechanistic role of Beclin 1 and its inhibition by prosurvival Bcl-2 family proteins

Bonsu Ku, Jae Sung Woo, Chengyu Liang, Kwang Hoon Lee, Jae U. Jung, Byung Ha Oh

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

A multiprotein complex composed of Beclin 1, PI(3)KC3 and UVRAG promotes autophagosome formation, while this activity is suppressed by a cohort of antiapoptotic Bcl-2 family members. Recently, we showed that a viral Bcl-2 of murine γ-herpesvirus 68, known as M11, binds to Beclin 1 with markedly high affinity in comparison with cellular Bcl-2 or Bcl-XL that interacts with Beclin 1 weakly.1 Furthermore, the binding affinity directly correlated with the potency of inhibition of autophagosome formation in cells. Herein, we present additional data showing that Beclin 1 forms a large homo-oligomer, and this oligomerization is partly disrupted by the binding of M11. Oligomerized Beclin 1 is proposed to serve as a platform enabling a concerted action of many molecules of the associating proteins, including Bif-1 that could be directly involved in autophagosome biogenesis on membranes owing to its BAR domain.

Original languageEnglish
Pages (from-to)519-520
Number of pages2
JournalAutophagy
Volume4
Issue number4
DOIs
Publication statusPublished - 2008 May 16
Externally publishedYes

Fingerprint

Proteins
Rhadinovirus
Multiprotein Complexes
Inhibition (Psychology)
Beclin-1
Membranes
Autophagosomes

Keywords

  • Autophagy
  • Bcl-2
  • Beclin 1
  • M11
  • Oligomerization

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

An insight into the mechanistic role of Beclin 1 and its inhibition by prosurvival Bcl-2 family proteins. / Ku, Bonsu; Woo, Jae Sung; Liang, Chengyu; Lee, Kwang Hoon; Jung, Jae U.; Oh, Byung Ha.

In: Autophagy, Vol. 4, No. 4, 16.05.2008, p. 519-520.

Research output: Contribution to journalArticle

Ku, Bonsu ; Woo, Jae Sung ; Liang, Chengyu ; Lee, Kwang Hoon ; Jung, Jae U. ; Oh, Byung Ha. / An insight into the mechanistic role of Beclin 1 and its inhibition by prosurvival Bcl-2 family proteins. In: Autophagy. 2008 ; Vol. 4, No. 4. pp. 519-520.
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