An integrated microfluidic culture device to regulate endothelial cell differentiation from embryonic stem cells

Jong Min Lee, Ji Eun Kim, Edward Kang, Sang Hoon Lee, Bong Geun Chung

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

We developed an integrated microfluidic culture device to regulate embryonic stem (ES) cell fate. The integrated microfluidic culture device consists of an air control channel and a fluidic channel with 4×4 micropillar arrays. We hypothesized that the microscale posts within the micropillar arrays would enable the control of uniform cell docking and shear stress profiles. We demonstrated that ES cells cultured for 6 days in the integrated microfluidic culture device differentiated into endothelial cells. Therefore, our integrated microfluidic culture device is a potentially powerful tool for directing ES cell fate.

Original languageEnglish
Pages (from-to)3133-3137
Number of pages5
JournalElectrophoresis
Volume32
Issue number22
DOIs
Publication statusPublished - 2011 Nov 1

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Lab-On-A-Chip Devices
Endothelial cells
Embryonic Stem Cells
Stem cells
Cell culture
Microfluidics
Cell Differentiation
Endothelial Cells
Fluidics
Shear stress
Air

Keywords

  • Embryonic stem cell
  • Endothelial cell differentiation
  • Integrated microfluidic device

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry

Cite this

An integrated microfluidic culture device to regulate endothelial cell differentiation from embryonic stem cells. / Lee, Jong Min; Kim, Ji Eun; Kang, Edward; Lee, Sang Hoon; Chung, Bong Geun.

In: Electrophoresis, Vol. 32, No. 22, 01.11.2011, p. 3133-3137.

Research output: Contribution to journalArticle

Lee, Jong Min ; Kim, Ji Eun ; Kang, Edward ; Lee, Sang Hoon ; Chung, Bong Geun. / An integrated microfluidic culture device to regulate endothelial cell differentiation from embryonic stem cells. In: Electrophoresis. 2011 ; Vol. 32, No. 22. pp. 3133-3137.
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