An integrated slidable direct polymerase chain reaction-capillary electrophoresis microdevice for rapid Y chromosome short tandem repeat analysis

Dohwan Lee, Yong Tae Kim, Jeewon Lee, Do Hyun Kim, Tae Seok Seo

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We have developed an integrated slidable direct polymerase chain reaction-capillary electrophoresis (Direct PCR-CE) microdevice to analyze mini-Y chromosome short tandem repeat (mini-Y STR) typing without a DNA purification step. The Direct PCR has been recently commercialized as a simple DNA amplification technique, which does not require any sample preparation steps such as cell lysis, DNA extraction and purification for amplifying specific target genes. By implementing the advantages of Direct PCR on a microdevice, we can amplify target min-Y STR loci directly from human whole blood in a micro-reactor (2μL) and the amplicons were successively separated by micro-capillary electrophoresis. The utilization of a slidable plate enables us to manually control the fluid without use of microvalves and microtubes, and the functional units of sample injection, Direct PCR, and CE analysis were sequentially and independently performed by switching the slidable plate to each unit. On the integrated slidable Direct PCR-CE microdevice, we could complete monoplex and multiplex mini-Y STR typing directly using human whole blood within 1 h.

Original languageEnglish
Pages (from-to)2644-2649
Number of pages6
JournalKorean Journal of Chemical Engineering
Volume33
Issue number9
DOIs
Publication statusPublished - 2016 Sep 1
Externally publishedYes

Fingerprint

Capillary electrophoresis
Polymerase chain reaction
Chromosomes
Microsatellite Repeats
DNA
Purification
Blood
Direct injection
Amplification
Genes
Fluids

Keywords

  • Capillary Electrophoresis
  • Direct Polymerase Chain Reaction
  • Microdevice
  • Mini-Y STR Typing

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

Cite this

An integrated slidable direct polymerase chain reaction-capillary electrophoresis microdevice for rapid Y chromosome short tandem repeat analysis. / Lee, Dohwan; Kim, Yong Tae; Lee, Jeewon; Kim, Do Hyun; Seo, Tae Seok.

In: Korean Journal of Chemical Engineering, Vol. 33, No. 9, 01.09.2016, p. 2644-2649.

Research output: Contribution to journalArticle

@article{45bd9994204f4375ad9b9acdf0e874e4,
title = "An integrated slidable direct polymerase chain reaction-capillary electrophoresis microdevice for rapid Y chromosome short tandem repeat analysis",
abstract = "We have developed an integrated slidable direct polymerase chain reaction-capillary electrophoresis (Direct PCR-CE) microdevice to analyze mini-Y chromosome short tandem repeat (mini-Y STR) typing without a DNA purification step. The Direct PCR has been recently commercialized as a simple DNA amplification technique, which does not require any sample preparation steps such as cell lysis, DNA extraction and purification for amplifying specific target genes. By implementing the advantages of Direct PCR on a microdevice, we can amplify target min-Y STR loci directly from human whole blood in a micro-reactor (2μL) and the amplicons were successively separated by micro-capillary electrophoresis. The utilization of a slidable plate enables us to manually control the fluid without use of microvalves and microtubes, and the functional units of sample injection, Direct PCR, and CE analysis were sequentially and independently performed by switching the slidable plate to each unit. On the integrated slidable Direct PCR-CE microdevice, we could complete monoplex and multiplex mini-Y STR typing directly using human whole blood within 1 h.",
keywords = "Capillary Electrophoresis, Direct Polymerase Chain Reaction, Microdevice, Mini-Y STR Typing",
author = "Dohwan Lee and Kim, {Yong Tae} and Jeewon Lee and Kim, {Do Hyun} and Seo, {Tae Seok}",
year = "2016",
month = "9",
day = "1",
doi = "10.1007/s11814-016-0103-9",
language = "English",
volume = "33",
pages = "2644--2649",
journal = "Korean Journal of Chemical Engineering",
issn = "0256-1115",
publisher = "Springer New York",
number = "9",

}

TY - JOUR

T1 - An integrated slidable direct polymerase chain reaction-capillary electrophoresis microdevice for rapid Y chromosome short tandem repeat analysis

AU - Lee, Dohwan

AU - Kim, Yong Tae

AU - Lee, Jeewon

AU - Kim, Do Hyun

AU - Seo, Tae Seok

PY - 2016/9/1

Y1 - 2016/9/1

N2 - We have developed an integrated slidable direct polymerase chain reaction-capillary electrophoresis (Direct PCR-CE) microdevice to analyze mini-Y chromosome short tandem repeat (mini-Y STR) typing without a DNA purification step. The Direct PCR has been recently commercialized as a simple DNA amplification technique, which does not require any sample preparation steps such as cell lysis, DNA extraction and purification for amplifying specific target genes. By implementing the advantages of Direct PCR on a microdevice, we can amplify target min-Y STR loci directly from human whole blood in a micro-reactor (2μL) and the amplicons were successively separated by micro-capillary electrophoresis. The utilization of a slidable plate enables us to manually control the fluid without use of microvalves and microtubes, and the functional units of sample injection, Direct PCR, and CE analysis were sequentially and independently performed by switching the slidable plate to each unit. On the integrated slidable Direct PCR-CE microdevice, we could complete monoplex and multiplex mini-Y STR typing directly using human whole blood within 1 h.

AB - We have developed an integrated slidable direct polymerase chain reaction-capillary electrophoresis (Direct PCR-CE) microdevice to analyze mini-Y chromosome short tandem repeat (mini-Y STR) typing without a DNA purification step. The Direct PCR has been recently commercialized as a simple DNA amplification technique, which does not require any sample preparation steps such as cell lysis, DNA extraction and purification for amplifying specific target genes. By implementing the advantages of Direct PCR on a microdevice, we can amplify target min-Y STR loci directly from human whole blood in a micro-reactor (2μL) and the amplicons were successively separated by micro-capillary electrophoresis. The utilization of a slidable plate enables us to manually control the fluid without use of microvalves and microtubes, and the functional units of sample injection, Direct PCR, and CE analysis were sequentially and independently performed by switching the slidable plate to each unit. On the integrated slidable Direct PCR-CE microdevice, we could complete monoplex and multiplex mini-Y STR typing directly using human whole blood within 1 h.

KW - Capillary Electrophoresis

KW - Direct Polymerase Chain Reaction

KW - Microdevice

KW - Mini-Y STR Typing

UR - http://www.scopus.com/inward/record.url?scp=84975128662&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84975128662&partnerID=8YFLogxK

U2 - 10.1007/s11814-016-0103-9

DO - 10.1007/s11814-016-0103-9

M3 - Article

AN - SCOPUS:84975128662

VL - 33

SP - 2644

EP - 2649

JO - Korean Journal of Chemical Engineering

JF - Korean Journal of Chemical Engineering

SN - 0256-1115

IS - 9

ER -