An ultra-sensitive detection of a whole virus using dual aptamers developed by immobilization-free screening

Jee Woong Park, Su Jin Lee, Eun Jin Choi, Jaejo Kim, Jae Young Song, Man Bock Gu

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

In this study, we successfully developed a ssDNA aptamer pairs by using an advanced immobilization-free SELEX method with affinity-based selection and counter-screening process at every round. By implementing this method, two different aptamers specifically binding to bovine viral diarrhea virus type 1(BVDV type 1) with high affinity were successfully screened. This aptamer pair was applied to ultrasensitive detection platform for BVDV type 1 in a sandwich manner. The ultrasensitive detection of BVDV type 1 using one of aptamers conjugated with gold nanoparticles was obtained in aptamer-aptamer sandwich type sensing format, with the limit of detection of 800. copies/ml, which is comparable to a real-time PCR method.

Original languageEnglish
Pages (from-to)324-329
Number of pages6
JournalBiosensors and Bioelectronics
Volume51
DOIs
Publication statusPublished - 2014 Jan 5

Keywords

  • Bovine viral diarrhea virus (BVDV)
  • Dual aptamers
  • SELEX
  • SPR
  • Sandwich

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

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