An Ultrasensitive FRET-based DNA Sensor via the Accumulated QD System Derivatized in the Nano-beads

Lan Hee Yang, Dong June Ahn, Eunhae Koo

Research output: Contribution to journalArticle

Abstract

Förster resonance energy transfer (FRET) is extremely sensitive to the separation distance between the donor and the acceptor which is ideal for probing such biological phenomena. Also, FRET-based probes have been developing for detecting an unamplified, low-abundance of target DNA. Here we describe the development of FRET based DNA sensor based on an accumulated QD system for detecting KRAS G12D mutation which is the most common mutation in cancer. The accumulated QD system consists of the polystyrene beads which surface is modified with carboxyl modified QDs. The QDs are sandwich-hybridized with DNA of a capture probe, a reporter probe with Texas-red, and a target DNA by EDC-NHS coupling. Because the carboxyl modified QDs are located closely to each other in the accumulated QDs, these neighboring QDs are enough to transfer the energy to the acceptor dyes. Therefore the FRET factor in the bead system is enhancing by the additional increase of 29.2% as compared to that in a single QD system. These results suggest that the accumulated nanobead probe with conjugated QDs can be used as ultrasensitive DNA nanosensors detecting the mutation in the various cancers.

Original languageEnglish
Pages (from-to)340-347
Number of pages8
JournalBiochip Journal
Volume12
Issue number4
DOIs
Publication statusPublished - 2018 Dec 1

Fingerprint

Energy Transfer
Energy transfer
DNA
Sensors
Mutation
Nanosensors
Biological Phenomena
Transfer Factor
Polystyrenes
Neoplasms
Coloring Agents
Dyes

Keywords

  • Bead
  • DNA sensor
  • FRET
  • QD

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Electrical and Electronic Engineering

Cite this

An Ultrasensitive FRET-based DNA Sensor via the Accumulated QD System Derivatized in the Nano-beads. / Yang, Lan Hee; Ahn, Dong June; Koo, Eunhae.

In: Biochip Journal, Vol. 12, No. 4, 01.12.2018, p. 340-347.

Research output: Contribution to journalArticle

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