Analyses of fruit flies that do not express selenoproteins or express the mouse selenoprotein, methionine sulfoxide reductase B1,reveal a role of selenoproteins in stress resistance

Valentina A. Shchedrina, Hadise Kabil, Gerd Vorbruggen, Byung Cheon Lee, An Ton A. Turanov, Mitsuko Hirosawa-Takamori, Hwa Young Kim, Lawrence G. Harshman, Dolph L. Hatfield, Vadim N. Gladyshev

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Selenoproteins are essential in vertebrates because of their crucial role in cellular redox homeostasis, but some invertebrates that lack selenoproteins have recently been identified. Genetic disruption of selenoprotein biosynthesis had no effect on lifespan and oxidative stress resistance of Drosophila melanogaster. In the current study, fruit flies with knock-out of the selenocysteine-specific elongation factor were metabolically labeled with 75Se; they did not incorporate selenium into proteins and had the same lifespan on a chemically defined diet with or without selenium supplementation. These flies were, however, more susceptible to starvation than controls, and this effect could be ascribed to the function of selenoprotein K. We further expressed mouse methionine sulfoxide reductase B1 (MsrB1), a selenoenzyme that catalyzes the reduction of oxidized methionine residues and has protein repair function, in the whole body or the nervous system of fruit flies. This exogenous selenoprotein could only be expressed when the Drosophila selenocysteine insertion sequence element was used, whereas the corresponding mouse element did not support selenoprotein synthesis. Ectopic expression of MsrB1 in the nervous system led to an increase in the resistance against oxidative stress and starvation, but did not affect lifespan and reproduction, whereas ubiquitous MsrB1 expression had no effect. Dietary selenium did not influence lifespan of MsrB1-expressing flies. Thus, in contrast to vertebrates, fruit flies preserve only three selenoproteins, which are not essential and play a role only under certain stress conditions, thereby limiting the use of the micronutrient selenium by these organisms.

Original languageEnglish
Pages (from-to)29449-29461
Number of pages13
JournalJournal of Biological Chemistry
Volume286
Issue number34
DOIs
Publication statusPublished - 2011 Aug 26
Externally publishedYes

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Selenoproteins
Fruits
Diptera
Fruit
Selenium
Selenocysteine
Oxidative stress
Neurology
Starvation
Nervous System
Vertebrates
Oxidative Stress
Peptide Elongation Factors
Formulated Food
DNA Transposable Elements
Micronutrients
Biosynthesis
Invertebrates
Nutrition
methionine sulfoxide reductase

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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Analyses of fruit flies that do not express selenoproteins or express the mouse selenoprotein, methionine sulfoxide reductase B1,reveal a role of selenoproteins in stress resistance. / Shchedrina, Valentina A.; Kabil, Hadise; Vorbruggen, Gerd; Lee, Byung Cheon; Turanov, An Ton A.; Hirosawa-Takamori, Mitsuko; Kim, Hwa Young; Harshman, Lawrence G.; Hatfield, Dolph L.; Gladyshev, Vadim N.

In: Journal of Biological Chemistry, Vol. 286, No. 34, 26.08.2011, p. 29449-29461.

Research output: Contribution to journalArticle

Shchedrina, VA, Kabil, H, Vorbruggen, G, Lee, BC, Turanov, ATA, Hirosawa-Takamori, M, Kim, HY, Harshman, LG, Hatfield, DL & Gladyshev, VN 2011, 'Analyses of fruit flies that do not express selenoproteins or express the mouse selenoprotein, methionine sulfoxide reductase B1,reveal a role of selenoproteins in stress resistance', Journal of Biological Chemistry, vol. 286, no. 34, pp. 29449-29461. https://doi.org/10.1074/jbc.M111.257600
Shchedrina, Valentina A. ; Kabil, Hadise ; Vorbruggen, Gerd ; Lee, Byung Cheon ; Turanov, An Ton A. ; Hirosawa-Takamori, Mitsuko ; Kim, Hwa Young ; Harshman, Lawrence G. ; Hatfield, Dolph L. ; Gladyshev, Vadim N. / Analyses of fruit flies that do not express selenoproteins or express the mouse selenoprotein, methionine sulfoxide reductase B1,reveal a role of selenoproteins in stress resistance. In: Journal of Biological Chemistry. 2011 ; Vol. 286, No. 34. pp. 29449-29461.
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