Analysis and characterization of hepatitis B vaccine particles synthesized from Hansenula polymorpha

Hyuk Seong Seo, Jin Seung Park, Kyung Yeon Han, Kyung Dong Bae, Sang Jeom Ahn, Hyun Ah Kang, Jeewon Lee

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

The biochemical and physical properties of hepatitis B virus (HBV) small surface antigen (S-HBVsAg) from Berna Biotech Korea Corp. were systematically analyzed and characterized. Through various electrophoresis and immunoblotting assay of S-HBVsAg and its proteolytic products, it was confirmed that the S-HBVsAg vaccine particles are present in the form of covalent multimers that are assembled via strong intermolecular disulfide bonds. The S-HBVsAg particles contain no N-glycosylation moiety but some O-glycosidically linked mannoses. Evidently from N-terminus sequencing of both monomers and dimers that are formed by complete and partial reduction, respectively, of the S-HBVsAg particles under reducing SDS-PAGE condition, it is evident that each polypeptide within S-HBVsAg particles has authentic sequence of N-terminus. Denaturation plot shows that the S-HBVsAg vaccine particles were extremely stable especially in the solution with high acidity. This stability property of S-HBVsAg vaccine particles could provide very useful information for the optimization of the downstream process of recombinant S-HBVsAg particles synthesized from yeast cultures.

Original languageEnglish
Pages (from-to)4138-4144
Number of pages7
JournalVaccine
Volume26
Issue number33
DOIs
Publication statusPublished - 2008 Aug 5

Keywords

  • Covalent multimer
  • Glycosylation
  • Hepatitis B surface antigen
  • Stability
  • Yeast

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

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