TY - JOUR
T1 - Analysis of alkali-soluble glucan produced by Saccharomyces cerevisiae wild-type and mutants
AU - Ha, C. H.
AU - Lim, K. H.
AU - Kim, Y. T.
AU - Lim, S. T.
AU - Kim, C. W.
AU - Chang, H. I.
N1 - Funding Information:
Acknowledgement This study was supported by Ministry of Health and Welfare, Republic of Korea, project No. HMP-97-D-4–0017, and we thank Professor Sang-Duk Kim for her careful advice.
PY - 2002
Y1 - 2002
N2 - The alkali-soluble glucan of the yeast cell wall contains β-(1,3)- and (1,6)-D-linkages and systemically enhances the immune system. To isolate Saccharomyces cerevisiae mutants producing glucan with a high degree of β-(1,6)-D-glycosidic bonds, a wild-type strain was mutagenized with ultraviolet light. The mutants were then selected by treatment with 1.0 mg laminarinase, endo-β-(1,3)-D-glucanase/ml. The alkali-soluble glucan was extracted by modified alkali ysis followed by the Cetavlon method and concanavalin-A chromatography. The prepared alkali-soluble glucans from the wild-type and the mutants were compared with respect to yield and polymer structure using gas chromatography, 13C-NMR spectrometry, high performance liquid, and multi-angle laser light scattering and refractive index detectors. The results indicated that the S. cerevisiae mutants had ten-fold more alkali-soluble glucan than the wild-type. Structural analysis revealed that the alkali-soluble glucan from the mutants also had a higher degree of β-(1,6)-D-linkage than that from the wild-type.
AB - The alkali-soluble glucan of the yeast cell wall contains β-(1,3)- and (1,6)-D-linkages and systemically enhances the immune system. To isolate Saccharomyces cerevisiae mutants producing glucan with a high degree of β-(1,6)-D-glycosidic bonds, a wild-type strain was mutagenized with ultraviolet light. The mutants were then selected by treatment with 1.0 mg laminarinase, endo-β-(1,3)-D-glucanase/ml. The alkali-soluble glucan was extracted by modified alkali ysis followed by the Cetavlon method and concanavalin-A chromatography. The prepared alkali-soluble glucans from the wild-type and the mutants were compared with respect to yield and polymer structure using gas chromatography, 13C-NMR spectrometry, high performance liquid, and multi-angle laser light scattering and refractive index detectors. The results indicated that the S. cerevisiae mutants had ten-fold more alkali-soluble glucan than the wild-type. Structural analysis revealed that the alkali-soluble glucan from the mutants also had a higher degree of β-(1,6)-D-linkage than that from the wild-type.
UR - http://www.scopus.com/inward/record.url?scp=0036191157&partnerID=8YFLogxK
U2 - 10.1007/s002530100824
DO - 10.1007/s002530100824
M3 - Article
C2 - 11935190
AN - SCOPUS:0036191157
SN - 0175-7598
VL - 58
SP - 370
EP - 377
JO - Applied Microbiology and Biotechnology
JF - Applied Microbiology and Biotechnology
IS - 3
ER -