Analysis of alkali-soluble glucan produced by Saccharomyces cerevisiae wild-type and mutants

C. H. Ha, K. H. Lim, Y. T. Kim, Seung Taik Lim, Chan Wha Kim, Hyo-Ihl Chang

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The alkali-soluble glucan of the yeast cell wall contains β-(1,3)- and (1,6)-D-linkages and systemically enhances the immune system. To isolate Saccharomyces cerevisiae mutants producing glucan with a high degree of β-(1,6)-D-glycosidic bonds, a wild-type strain was mutagenized with ultraviolet light. The mutants were then selected by treatment with 1.0 mg laminarinase, endo-β-(1,3)-D-glucanase/ml. The alkali-soluble glucan was extracted by modified alkali ysis followed by the Cetavlon method and concanavalin-A chromatography. The prepared alkali-soluble glucans from the wild-type and the mutants were compared with respect to yield and polymer structure using gas chromatography, 13C-NMR spectrometry, high performance liquid, and multi-angle laser light scattering and refractive index detectors. The results indicated that the S. cerevisiae mutants had ten-fold more alkali-soluble glucan than the wild-type. Structural analysis revealed that the alkali-soluble glucan from the mutants also had a higher degree of β-(1,6)-D-linkage than that from the wild-type.

Original languageEnglish
Pages (from-to)370-377
Number of pages8
JournalApplied Microbiology and Biotechnology
Volume58
Issue number3
DOIs
Publication statusPublished - 2002 Mar 11

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Glucans
Alkalies
Yeast
Saccharomyces cerevisiae
Immune system
Chromatography
Structural analysis
Gas chromatography
Light scattering
Spectrometry
Refractive index
Cells
Nuclear magnetic resonance
Detectors
Cellulases
Refractometry
Lasers
Liquids
Polymers
Ultraviolet Rays

ASJC Scopus subject areas

  • Biotechnology
  • Microbiology
  • Bioengineering

Cite this

Analysis of alkali-soluble glucan produced by Saccharomyces cerevisiae wild-type and mutants. / Ha, C. H.; Lim, K. H.; Kim, Y. T.; Lim, Seung Taik; Kim, Chan Wha; Chang, Hyo-Ihl.

In: Applied Microbiology and Biotechnology, Vol. 58, No. 3, 11.03.2002, p. 370-377.

Research output: Contribution to journalArticle

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