Analysis of C5a-mediated chemotaxis by lentiviral delivery of small interfering RNA

Jong-Ik Hwang, Iain D C Fraser, Sangdun Choi, Xiao Feng Qin, Melvin I. Simon

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Immune cells respond to chemotactic signals by means of G protein-coupled receptors. Attempts to elucidate the function of specific G protein family members in these responses is complicated by redundancy among the different G protein isoforms. We have used lentiviral-based RNA interference to eliminate expression of specific G protein subunits selectively in J774A.1 mouse macrophages. The chemotactic response to the complement factors C5a and C3a is ablated in cells lacking Gβ2 but is unaffected in cells lacking Gβ 1, Gαi 2, or Gαi 3. Similarly, the C5a-mediated calcium response of single cells is either absent or significantly delayed and weakened by Gβ2 knockdown. Assessment of Akt1 phosphorylation levels in response to C5a shows rapid and sustained phosphorylation in both wild-type cells and cells lacking Gβ 1. Cells lacking Gβ 2 retain the rapid response but cannot sustain phospho-Akt1 levels. The phenotype of cells lacking Gβ 2 can be reversed by overexpression of either human Gβ 2 or mouse Gβ 1. These data demonstrate the usefulness of lentiviral-based RNA interference in the systematic analysis of a signaling pathway, and they suggest that in J774A.1 cells, Gβ 2-derived Gβγ is the most effective mediator of chemotaxis to C5a.

Original languageEnglish
Pages (from-to)488-493
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume101
Issue number2
DOIs
Publication statusPublished - 2004 Jan 13
Externally publishedYes

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Gastrin-Secreting Cells
Chemotaxis
Small Interfering RNA
GTP-Binding Proteins
RNA Interference
Complement C3a
Complement C5a
Phosphorylation
Protein Subunits
G-Protein-Coupled Receptors
Protein Isoforms
Macrophages
Calcium
Phenotype

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Analysis of C5a-mediated chemotaxis by lentiviral delivery of small interfering RNA. / Hwang, Jong-Ik; Fraser, Iain D C; Choi, Sangdun; Qin, Xiao Feng; Simon, Melvin I.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, No. 2, 13.01.2004, p. 488-493.

Research output: Contribution to journalArticle

Hwang, Jong-Ik ; Fraser, Iain D C ; Choi, Sangdun ; Qin, Xiao Feng ; Simon, Melvin I. / Analysis of C5a-mediated chemotaxis by lentiviral delivery of small interfering RNA. In: Proceedings of the National Academy of Sciences of the United States of America. 2004 ; Vol. 101, No. 2. pp. 488-493.
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