Analysis of cattle olfactory subgenome: The first detail study on the characteristics of the complete olfactory receptor repertoire of a ruminant

Kyooyeol Lee, Dinh Truong Nguyen, Minkyeung Choi, Se Yeoun Cha, Jin Hoi Kim, Hailu Dadi, Han Geuk Seo, Kunho Seo, Taehoon Chun, Chankyu Park

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Background: Mammalian olfactory receptors (ORs) are encoded by the largest mammalian multigene family. Understanding the OR gene repertoire in the cattle genome could lead to link the effects of genetic differences in these genes to variations in olfaction in cattle.Results: We report here a whole genome analysis of the olfactory receptor genes of Bos taurus using conserved OR gene-specific motifs and known OR protein sequences from diverse species. Our analysis, using the current cattle genome assembly UMD 3.1 covering 99.9% of the cattle genome, shows that the cattle genome contains 1,071 OR-related sequences including 881 functional, 190 pseudo, and 352 partial OR sequences. The OR genes are located in 49 clusters on 26 cattle chromosomes. We classified them into 18 families consisting of 4 Class I and 14 Class II families and these were further grouped into 272 subfamilies. Comparative analyses of the OR genes of cattle, pigs, humans, mice, and dogs showed that 6.0% (n = 53) of functional OR cattle genes were species-specific. We also showed that significant copy number variations are present in the OR repertoire of the cattle from the analysis of 10 selected OR genes.Conclusion: Our analysis revealed the almost complete OR gene repertoire from an individual cattle genome. Though the number of OR genes were lower than in pigs, the analysis of the genetic system of cattle ORs showed close similarities to that of the pig.

Original languageEnglish
Article number596
JournalBMC Genomics
Volume14
Issue number1
DOIs
Publication statusPublished - 2013 Sep 2

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Odorant Receptors
Ruminants
Genes
Genome
Swine
Smell

Keywords

  • Cattle
  • Olfaction
  • Olfactory receptor
  • OR genes

ASJC Scopus subject areas

  • Biotechnology
  • Genetics

Cite this

Analysis of cattle olfactory subgenome : The first detail study on the characteristics of the complete olfactory receptor repertoire of a ruminant. / Lee, Kyooyeol; Nguyen, Dinh Truong; Choi, Minkyeung; Cha, Se Yeoun; Kim, Jin Hoi; Dadi, Hailu; Seo, Han Geuk; Seo, Kunho; Chun, Taehoon; Park, Chankyu.

In: BMC Genomics, Vol. 14, No. 1, 596, 02.09.2013.

Research output: Contribution to journalArticle

Lee, Kyooyeol ; Nguyen, Dinh Truong ; Choi, Minkyeung ; Cha, Se Yeoun ; Kim, Jin Hoi ; Dadi, Hailu ; Seo, Han Geuk ; Seo, Kunho ; Chun, Taehoon ; Park, Chankyu. / Analysis of cattle olfactory subgenome : The first detail study on the characteristics of the complete olfactory receptor repertoire of a ruminant. In: BMC Genomics. 2013 ; Vol. 14, No. 1.
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