Analysis of cell growth in three-dimensional scaffolds

James C Y Dunn, Wan Yin Chan, Vittorio Cristini, Junseok Kim, John Lowengrub, Shivani Singh, Benjamin M. Wu

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

The in vitro growth of pre-osteoblasts in multi-layer, three-dimensional scaffolds was determined from experimental measurements and was compared to a mathematical model. Immediately following cell seeding, the initial cell density was uniform throughout the scaffold. After 10 days, the cell density increased from 2.1 × 105 cells/cm3 to 1.3 × 10 7 cells/cm3 at the fluid-scaffold interface. The increase in cell density was largely confined to the outermost 200 μm from the fluid-scaffold interface. The cell density profile was in good agreement with a mathematical model that simulated the cell growth based on the local oxygen tension. The improved understanding derived from this mathematical model may be useful in the design of three-dimensional scaffolds that can support more uniform growth of cells.

Original languageEnglish
Pages (from-to)705-716
Number of pages12
JournalTissue Engineering
Volume12
Issue number4
DOIs
Publication statusPublished - 2006 Apr 1
Externally publishedYes

Fingerprint

Cell growth
Scaffolds (biology)
Scaffolds
Cell Count
Growth
Theoretical Models
Mathematical models
Fluids
Osteoblasts
Cells
Oxygen

ASJC Scopus subject areas

  • Biophysics
  • Cell Biology
  • Biotechnology

Cite this

Dunn, J. C. Y., Chan, W. Y., Cristini, V., Kim, J., Lowengrub, J., Singh, S., & Wu, B. M. (2006). Analysis of cell growth in three-dimensional scaffolds. Tissue Engineering, 12(4), 705-716. https://doi.org/10.1089/ten.2006.12.705

Analysis of cell growth in three-dimensional scaffolds. / Dunn, James C Y; Chan, Wan Yin; Cristini, Vittorio; Kim, Junseok; Lowengrub, John; Singh, Shivani; Wu, Benjamin M.

In: Tissue Engineering, Vol. 12, No. 4, 01.04.2006, p. 705-716.

Research output: Contribution to journalArticle

Dunn, JCY, Chan, WY, Cristini, V, Kim, J, Lowengrub, J, Singh, S & Wu, BM 2006, 'Analysis of cell growth in three-dimensional scaffolds', Tissue Engineering, vol. 12, no. 4, pp. 705-716. https://doi.org/10.1089/ten.2006.12.705
Dunn JCY, Chan WY, Cristini V, Kim J, Lowengrub J, Singh S et al. Analysis of cell growth in three-dimensional scaffolds. Tissue Engineering. 2006 Apr 1;12(4):705-716. https://doi.org/10.1089/ten.2006.12.705
Dunn, James C Y ; Chan, Wan Yin ; Cristini, Vittorio ; Kim, Junseok ; Lowengrub, John ; Singh, Shivani ; Wu, Benjamin M. / Analysis of cell growth in three-dimensional scaffolds. In: Tissue Engineering. 2006 ; Vol. 12, No. 4. pp. 705-716.
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