Analysis of cell growth in three-dimensional scaffolds

James C Y Dunn, Wan Yin Chan, Vittorio Cristini, Junseok Kim, John Lowengrub, Shivani Singh, Benjamin M. Wu

Research output: Contribution to journalArticle

98 Citations (Scopus)


The in vitro growth of pre-osteoblasts in multi-layer, three-dimensional scaffolds was determined from experimental measurements and was compared to a mathematical model. Immediately following cell seeding, the initial cell density was uniform throughout the scaffold. After 10 days, the cell density increased from 2.1 × 105 cells/cm3 to 1.3 × 10 7 cells/cm3 at the fluid-scaffold interface. The increase in cell density was largely confined to the outermost 200 μm from the fluid-scaffold interface. The cell density profile was in good agreement with a mathematical model that simulated the cell growth based on the local oxygen tension. The improved understanding derived from this mathematical model may be useful in the design of three-dimensional scaffolds that can support more uniform growth of cells.

Original languageEnglish
Pages (from-to)705-716
Number of pages12
JournalTissue Engineering
Issue number4
Publication statusPublished - 2006 Apr 1
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Cell Biology
  • Biotechnology

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    Dunn, J. C. Y., Chan, W. Y., Cristini, V., Kim, J., Lowengrub, J., Singh, S., & Wu, B. M. (2006). Analysis of cell growth in three-dimensional scaffolds. Tissue Engineering, 12(4), 705-716.