Analysis of cell separation efficiency in dielectrophoresis-activated cell sorter

Jaemin An, Jangwon Lee, Youngho Kim, Byungkyu Kim, Sangho Lee

Research output: Chapter in Book/Report/Conference proceedingConference contribution

7 Citations (Scopus)

Abstract

We investigate the key parameters that influence the efficiency of the previously presented dielectrophoresis(DEP)-activated cell sorter (DACS). While the conventional cell separators typically sort cells depending on the different sizes of cells, our system differentiates similarly-sized cells by responding to the variations of electrical properties among cells namely conductivity and permittivity. Thus, continuously varying DEP forces generated by the system enable us to separate MCF10A (normal cells derived from human breast epithelium) and MCF7 (human breast cancer cells) cells of similar size. Unfortunately, however, both cell types possess a wide range of cell size variation. In previous study, therefore, separation efficiency was limited to 77±5% for sorting MCF10A cells and MCF7 cells. To analyze separation efficiency according to cell size, we calculate cell size distributions before and after separation and then analyze the separation efficiency of cells according to cell size. Although cell separation is performed relying on the electrical properties of the cell, we conclude that cell size remains one of the key parameter that determines the efficiency of cell separation.

Original languageEnglish
Title of host publication3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2008
PublisherIEEE Computer Society
Pages965-969
Number of pages5
ISBN (Print)9781424419081
DOIs
Publication statusPublished - 2008
Event3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2008 - Sanya, China
Duration: 2008 Jan 62008 Jan 9

Publication series

Name3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS

Other

Other3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2008
CountryChina
CitySanya
Period08/1/608/1/9

Keywords

  • Conductivity
  • DACS
  • Dielectrophoresis(DEP)
  • MCF10A
  • MCF7
  • Permittivity

ASJC Scopus subject areas

  • Renewable Energy, Sustainability and the Environment
  • Management, Monitoring, Policy and Law
  • Energy(all)
  • Energy Engineering and Power Technology
  • Modelling and Simulation
  • Fuel Technology
  • Control and Systems Engineering
  • Electrical and Electronic Engineering

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  • Cite this

    An, J., Lee, J., Kim, Y., Kim, B., & Lee, S. (2008). Analysis of cell separation efficiency in dielectrophoresis-activated cell sorter. In 3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2008 (pp. 965-969). [4484482] (3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS). IEEE Computer Society. https://doi.org/10.1109/NEMS.2008.4484482