TY - GEN
T1 - Analysis of cell separation efficiency in dielectrophoresis-activated cell sorter
AU - An, Jaemin
AU - Lee, Jangwon
AU - Kim, Youngho
AU - Kim, Byungkyu
AU - Lee, Sangho
PY - 2008
Y1 - 2008
N2 - We investigate the key parameters that influence the efficiency of the previously presented dielectrophoresis(DEP)-activated cell sorter (DACS). While the conventional cell separators typically sort cells depending on the different sizes of cells, our system differentiates similarly-sized cells by responding to the variations of electrical properties among cells namely conductivity and permittivity. Thus, continuously varying DEP forces generated by the system enable us to separate MCF10A (normal cells derived from human breast epithelium) and MCF7 (human breast cancer cells) cells of similar size. Unfortunately, however, both cell types possess a wide range of cell size variation. In previous study, therefore, separation efficiency was limited to 77±5% for sorting MCF10A cells and MCF7 cells. To analyze separation efficiency according to cell size, we calculate cell size distributions before and after separation and then analyze the separation efficiency of cells according to cell size. Although cell separation is performed relying on the electrical properties of the cell, we conclude that cell size remains one of the key parameter that determines the efficiency of cell separation.
AB - We investigate the key parameters that influence the efficiency of the previously presented dielectrophoresis(DEP)-activated cell sorter (DACS). While the conventional cell separators typically sort cells depending on the different sizes of cells, our system differentiates similarly-sized cells by responding to the variations of electrical properties among cells namely conductivity and permittivity. Thus, continuously varying DEP forces generated by the system enable us to separate MCF10A (normal cells derived from human breast epithelium) and MCF7 (human breast cancer cells) cells of similar size. Unfortunately, however, both cell types possess a wide range of cell size variation. In previous study, therefore, separation efficiency was limited to 77±5% for sorting MCF10A cells and MCF7 cells. To analyze separation efficiency according to cell size, we calculate cell size distributions before and after separation and then analyze the separation efficiency of cells according to cell size. Although cell separation is performed relying on the electrical properties of the cell, we conclude that cell size remains one of the key parameter that determines the efficiency of cell separation.
KW - Conductivity
KW - DACS
KW - Dielectrophoresis(DEP)
KW - MCF10A
KW - MCF7
KW - Permittivity
UR - http://www.scopus.com/inward/record.url?scp=50249101709&partnerID=8YFLogxK
U2 - 10.1109/NEMS.2008.4484482
DO - 10.1109/NEMS.2008.4484482
M3 - Conference contribution
AN - SCOPUS:50249101709
SN - 9781424419081
T3 - 3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS
SP - 965
EP - 969
BT - 3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2008
PB - IEEE Computer Society
T2 - 3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2008
Y2 - 6 January 2008 through 9 January 2008
ER -
