BSouth Korea (HBV) DNA Korean Source HBV South Korea BSouth Korea S Korean Source

Translated title of the contribution: Analysis of clinical characteristics and S gene mutation of hepatitis B virus (HBV) in patients with hepatitis B surface antigen RIA negative and HBV DNA positive

Yong Hak Sohn, Heung Bum Oh, Sun-Young Ko, Young Suk Lim, Oh Joong Kwon

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Background: We investigated hepatitis B virus (HBV) infection cases, who were HBsAg negative by radioimmunoassay (RIA) and HBV DNA positive for their clinical characteristics, the S gene mutation of hepatitis B virus (HBV), and usefulness of other HBsAg immunoassay. Methods: Among the patients requested for HBV DNA quantification, 16 patients positive in HBV DNA but negative in HBsAg RIA (BNIBT HBsAg Kit, China) were enrolled. The "a" determinant of HBV S gene was sequenced and clinical characteristics were reviewed. Additional HBsAg assay was performed using Architect HBsAg kit (Abbott laboratories, USA) employing chemiluminescent immunoassay method. Results: Eleven of the 16 patients showed multiple mutations in the "a" determinant. These patients received liver transplantation several years ago and have been treated with hepatitis B immune globulin (HBIG) and antiviral drugs. G145R mutation was found in 8 patients and G145K, D144G, and D144A were also frequently found. Among 9 of the 11 patients tested for HBsAg by Architect HBs-Ag kit, 8 showed positive results. Among 4 of the remaining 5 patients, only 2 showed weak positive results (≤1 IU/mL) in Architect HBsAg kit. Conclusions: HBV DNA-positive/HBsAg RIA-negative results were mostly observed in the patients treated with HBIG after liver transplantation, in whom HBIG escape mutations were found. Majority of these cases were positive in Architect HBsAg assay, and it is recommended to use other HBsAg immunoassay methods that are more sensitive than RIA in the detection limit as well as in the detection of escape mutant in hospitals performing liver transplantation.

Original languageKorean
Pages (from-to)224-230
Number of pages7
JournalKorean Journal of Laboratory Medicine
Volume29
Issue number3
DOIs
Publication statusPublished - 2009 Oct 5
Externally publishedYes

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Hepatitis B Surface Antigens
Viruses
Hepatitis B virus
Radioimmunoassay
Genes
Mutation
DNA
Hepatitis B
Immunoassay
Liver
Liver Transplantation
Immunoglobulins
Assays
Virus Diseases
Antiviral Agents
Limit of Detection
China

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

BSouth Korea (HBV) DNA Korean Source HBV South Korea BSouth Korea S Korean Source. / Sohn, Yong Hak; Oh, Heung Bum; Ko, Sun-Young; Lim, Young Suk; Kwon, Oh Joong.

In: Korean Journal of Laboratory Medicine, Vol. 29, No. 3, 05.10.2009, p. 224-230.

Research output: Contribution to journalArticle

Sohn, Yong Hak ; Oh, Heung Bum ; Ko, Sun-Young ; Lim, Young Suk ; Kwon, Oh Joong. / BSouth Korea (HBV) DNA Korean Source HBV South Korea BSouth Korea S Korean Source. In: Korean Journal of Laboratory Medicine. 2009 ; Vol. 29, No. 3. pp. 224-230.
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abstract = "Background: We investigated hepatitis B virus (HBV) infection cases, who were HBsAg negative by radioimmunoassay (RIA) and HBV DNA positive for their clinical characteristics, the S gene mutation of hepatitis B virus (HBV), and usefulness of other HBsAg immunoassay. Methods: Among the patients requested for HBV DNA quantification, 16 patients positive in HBV DNA but negative in HBsAg RIA (BNIBT HBsAg Kit, China) were enrolled. The {"}a{"} determinant of HBV S gene was sequenced and clinical characteristics were reviewed. Additional HBsAg assay was performed using Architect HBsAg kit (Abbott laboratories, USA) employing chemiluminescent immunoassay method. Results: Eleven of the 16 patients showed multiple mutations in the {"}a{"} determinant. These patients received liver transplantation several years ago and have been treated with hepatitis B immune globulin (HBIG) and antiviral drugs. G145R mutation was found in 8 patients and G145K, D144G, and D144A were also frequently found. Among 9 of the 11 patients tested for HBsAg by Architect HBs-Ag kit, 8 showed positive results. Among 4 of the remaining 5 patients, only 2 showed weak positive results (≤1 IU/mL) in Architect HBsAg kit. Conclusions: HBV DNA-positive/HBsAg RIA-negative results were mostly observed in the patients treated with HBIG after liver transplantation, in whom HBIG escape mutations were found. Majority of these cases were positive in Architect HBsAg assay, and it is recommended to use other HBsAg immunoassay methods that are more sensitive than RIA in the detection limit as well as in the detection of escape mutant in hospitals performing liver transplantation.",
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KW - HBsAg assay

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KW - Occult hepatitis B virus infection

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