Abstract
Suppression subtractive hybridization was used to construct a subtracted library (ogfw) from plants of a wild rice species, Oryza grandiglumis, subjected to a fungal elicitor and physical wounding. To screen the differentially expressed transcripts in the library, we applied a reverse Northern blot analysis to a cDNA microarray containing 1,152 random clones. Based on the average expression ratio, we selected 156 clones showing an elevated expression level. The elevated expression levels and overall expression profiles over time were verified by Northern blot analysis. A comparative functional categorization of the subtracted expressed sequence tags (ESTs) of the ogfw library against ESTs isolated from blast-infected O. sativa showed that the functional categories of cell rescue, defense and virulence, transcription, and cellular transport and transport mechanism of the ogfw library were threefold higher in the former than in the latter. These subtracted ESTs can be presumed to be related to the defense/resistance system and will be used to investigate the defense mechanisms of wild rice and to provide new insights into the genome of wild rice, which in turn will assist molecular breeding strategies of cultivated rice.
| Original language | English |
|---|---|
| Pages (from-to) | 347-354 |
| Number of pages | 8 |
| Journal | Journal of Plant Research |
| Volume | 118 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 2005 Oct 1 |
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Keywords
- ESTs
- Fungal elicitor
- Microarray
- Oryza grandiglumis
- Suppression subtractive hybridization
- Wild rice
- Wound
ASJC Scopus subject areas
- Plant Science
Cite this
Analysis of differentially expressed transcripts of fungal elicitor- and wound-treated wild rice (Oryza grandiglumis). / Kim, Kyung Mi; Cho, Sung Ki; Shin, Sang Hyun; Kim, Gyung Tae; Lee, Jai Heon; Oh, Boung Jun; Kang, Kyung Ho; Hong, Jong Chan; Choi, Jun Young; Shin, Jeong Sheop; Chung, Young Soo.
In: Journal of Plant Research, Vol. 118, No. 5, 01.10.2005, p. 347-354.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Analysis of differentially expressed transcripts of fungal elicitor- and wound-treated wild rice (Oryza grandiglumis)
AU - Kim, Kyung Mi
AU - Cho, Sung Ki
AU - Shin, Sang Hyun
AU - Kim, Gyung Tae
AU - Lee, Jai Heon
AU - Oh, Boung Jun
AU - Kang, Kyung Ho
AU - Hong, Jong Chan
AU - Choi, Jun Young
AU - Shin, Jeong Sheop
AU - Chung, Young Soo
PY - 2005/10/1
Y1 - 2005/10/1
N2 - Suppression subtractive hybridization was used to construct a subtracted library (ogfw) from plants of a wild rice species, Oryza grandiglumis, subjected to a fungal elicitor and physical wounding. To screen the differentially expressed transcripts in the library, we applied a reverse Northern blot analysis to a cDNA microarray containing 1,152 random clones. Based on the average expression ratio, we selected 156 clones showing an elevated expression level. The elevated expression levels and overall expression profiles over time were verified by Northern blot analysis. A comparative functional categorization of the subtracted expressed sequence tags (ESTs) of the ogfw library against ESTs isolated from blast-infected O. sativa showed that the functional categories of cell rescue, defense and virulence, transcription, and cellular transport and transport mechanism of the ogfw library were threefold higher in the former than in the latter. These subtracted ESTs can be presumed to be related to the defense/resistance system and will be used to investigate the defense mechanisms of wild rice and to provide new insights into the genome of wild rice, which in turn will assist molecular breeding strategies of cultivated rice.
AB - Suppression subtractive hybridization was used to construct a subtracted library (ogfw) from plants of a wild rice species, Oryza grandiglumis, subjected to a fungal elicitor and physical wounding. To screen the differentially expressed transcripts in the library, we applied a reverse Northern blot analysis to a cDNA microarray containing 1,152 random clones. Based on the average expression ratio, we selected 156 clones showing an elevated expression level. The elevated expression levels and overall expression profiles over time were verified by Northern blot analysis. A comparative functional categorization of the subtracted expressed sequence tags (ESTs) of the ogfw library against ESTs isolated from blast-infected O. sativa showed that the functional categories of cell rescue, defense and virulence, transcription, and cellular transport and transport mechanism of the ogfw library were threefold higher in the former than in the latter. These subtracted ESTs can be presumed to be related to the defense/resistance system and will be used to investigate the defense mechanisms of wild rice and to provide new insights into the genome of wild rice, which in turn will assist molecular breeding strategies of cultivated rice.
KW - ESTs
KW - Fungal elicitor
KW - Microarray
KW - Oryza grandiglumis
KW - Suppression subtractive hybridization
KW - Wild rice
KW - Wound
UR - http://www.scopus.com/inward/record.url?scp=28444484635&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=28444484635&partnerID=8YFLogxK
U2 - 10.1007/s10265-005-0228-0
DO - 10.1007/s10265-005-0228-0
M3 - Article
C2 - 16136361
AN - SCOPUS:28444484635
VL - 118
SP - 347
EP - 354
JO - Journal of Plant Research
JF - Journal of Plant Research
SN - 0918-9440
IS - 5
ER -