Analysis of differentially regulated proteins in TM4 cells treated with bisphenol A

Do Youn Lee, Sang Soo Lee, Won A. Joo, Eun Ju Lee, Chan Wha Kim

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

BPA, bisphenol A, a monomer of epoxy resins and polycarbonate plastic, is used in many consumer products including the plastic linings of cans for food and babies' bottles. BPA has been reported to cause reproductive toxicity and affects cells in rats and mice at high doses. In this study, the effect of BPA on protein expression in TM4 cells (a mouse Sertoli cell line) known to play an essential role in Spermatogenesis was investigated by two-dimensional electrophoresis (2-DE). After 16 h exposure to 50, 100, 150, 200, and 250 μM of BPA, the viability of TM4 cells decreased to about 90, 85, 78, 55, and 30% of control respectively. Approximately 800 protein spots in TM4 cells were analyzed by 2-DE with pH 4-7 linear immobilized pH gradient (IPG) Dry Strip, and 11 proteins which showed significantly different expression levels were identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS). Among these, HSP 27 and placental calcium binding protein may be proteins differentially expressed by BPA exposure.

Original languageEnglish
Pages (from-to)1201-1208
Number of pages8
JournalBioscience, Biotechnology and Biochemistry
Volume68
Issue number6
DOIs
Publication statusPublished - 2004 Jun

Keywords

  • 2D gel electrohphoresis
  • Bisphenol A
  • Calcium binding protein
  • HSP 27
  • Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDITOF MS)
  • TM 4 cell

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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