Analysis of four pesticides, isoproturon, phenmedipham, pyridate and nitenpyram residues by high-performance liquid chromatography with diode-array detector

Sung Yong Yang, Yun Chang Koo, Zeng Wang, Kyeong Heo, Hyeongkook Kim, Eun Mi An, Han Seung Shin, Jin Won Lee, Kwang Won Lee

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A method for the determination of four pesticide compounds, urea (isoproturon), bis-carbamate (phenmedipham), thiocarbamate (pyridate) and vinyllidenediamine (nitenpyram) were examined and analyzed by HPLC with C-18 column (250 mm×4.6 mm, 5 μm diameter particle size). Mobile phase consisted of deionized water, acetonitrile and 50 mM KH2PO4 (pH 2.5). Isoproturon and phenmedipham analytical condition was isocratic elution of the column with 50% solvent A (acetonitrile) and 50% solvent B (deionized water); pyridate was 85% solvent A (acetonitrile) and 15% solvent B (deionized water) at a flow rate of 1 mL/min; and nitenpyram analytical condition was 90% solvent A (50 mM KH 2PO 4, pH 2.5) and 10% solvent B (acetonitrile) at a flow rate of 1 mL/min. In results, retention times were 6.12, 8.63, 9.40 and 12.76 min for isoproturon, phenmedipham, pyridate and nitenpyram, respectively. All injection volumes were 10 μL and the limit of quantitation was 0.05 mg/kg for four pesticide compounds, respectively. Recovery rate test was performed with three farm products, rice, apple and soybean. Four pesticide compounds were spiked at concentrations of 0.05, 0.1 and 0.5 mg/kg. The recovery rates were ranged from 70.18% to 118.08% and the standard deviations of all experiments were within 10%.

Original languageEnglish
Pages (from-to)1165-1170
Number of pages6
JournalJournal of the Korean Society of Food Science and Nutrition
Volume39
Issue number8
DOIs
Publication statusPublished - 2010 Dec 1

Fingerprint

pyridate
nitenpyram
phenmedipham
isoproturon
Pesticides
detectors
pesticides
high performance liquid chromatography
High Pressure Liquid Chromatography
Water
Thiocarbamates
thiocarbamates
Carbamates
water
carbamates
Malus
agricultural products
Soybeans
Particle Size
Urea

Keywords

  • Analysis
  • HPLC
  • Isoproturon
  • Nitenpyram
  • Pesticide
  • Phenmedipham
  • Pyridate
  • Residue

ASJC Scopus subject areas

  • Nutrition and Dietetics
  • Food Science

Cite this

Analysis of four pesticides, isoproturon, phenmedipham, pyridate and nitenpyram residues by high-performance liquid chromatography with diode-array detector. / Yang, Sung Yong; Koo, Yun Chang; Wang, Zeng; Heo, Kyeong; Kim, Hyeongkook; An, Eun Mi; Shin, Han Seung; Lee, Jin Won; Lee, Kwang Won.

In: Journal of the Korean Society of Food Science and Nutrition, Vol. 39, No. 8, 01.12.2010, p. 1165-1170.

Research output: Contribution to journalArticle

Yang, Sung Yong ; Koo, Yun Chang ; Wang, Zeng ; Heo, Kyeong ; Kim, Hyeongkook ; An, Eun Mi ; Shin, Han Seung ; Lee, Jin Won ; Lee, Kwang Won. / Analysis of four pesticides, isoproturon, phenmedipham, pyridate and nitenpyram residues by high-performance liquid chromatography with diode-array detector. In: Journal of the Korean Society of Food Science and Nutrition. 2010 ; Vol. 39, No. 8. pp. 1165-1170.
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abstract = "A method for the determination of four pesticide compounds, urea (isoproturon), bis-carbamate (phenmedipham), thiocarbamate (pyridate) and vinyllidenediamine (nitenpyram) were examined and analyzed by HPLC with C-18 column (250 mm×4.6 mm, 5 μm diameter particle size). Mobile phase consisted of deionized water, acetonitrile and 50 mM KH2PO4 (pH 2.5). Isoproturon and phenmedipham analytical condition was isocratic elution of the column with 50{\%} solvent A (acetonitrile) and 50{\%} solvent B (deionized water); pyridate was 85{\%} solvent A (acetonitrile) and 15{\%} solvent B (deionized water) at a flow rate of 1 mL/min; and nitenpyram analytical condition was 90{\%} solvent A (50 mM KH 2PO 4, pH 2.5) and 10{\%} solvent B (acetonitrile) at a flow rate of 1 mL/min. In results, retention times were 6.12, 8.63, 9.40 and 12.76 min for isoproturon, phenmedipham, pyridate and nitenpyram, respectively. All injection volumes were 10 μL and the limit of quantitation was 0.05 mg/kg for four pesticide compounds, respectively. Recovery rate test was performed with three farm products, rice, apple and soybean. Four pesticide compounds were spiked at concentrations of 0.05, 0.1 and 0.5 mg/kg. The recovery rates were ranged from 70.18{\%} to 118.08{\%} and the standard deviations of all experiments were within 10{\%}.",
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T1 - Analysis of four pesticides, isoproturon, phenmedipham, pyridate and nitenpyram residues by high-performance liquid chromatography with diode-array detector

AU - Yang, Sung Yong

AU - Koo, Yun Chang

AU - Wang, Zeng

AU - Heo, Kyeong

AU - Kim, Hyeongkook

AU - An, Eun Mi

AU - Shin, Han Seung

AU - Lee, Jin Won

AU - Lee, Kwang Won

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N2 - A method for the determination of four pesticide compounds, urea (isoproturon), bis-carbamate (phenmedipham), thiocarbamate (pyridate) and vinyllidenediamine (nitenpyram) were examined and analyzed by HPLC with C-18 column (250 mm×4.6 mm, 5 μm diameter particle size). Mobile phase consisted of deionized water, acetonitrile and 50 mM KH2PO4 (pH 2.5). Isoproturon and phenmedipham analytical condition was isocratic elution of the column with 50% solvent A (acetonitrile) and 50% solvent B (deionized water); pyridate was 85% solvent A (acetonitrile) and 15% solvent B (deionized water) at a flow rate of 1 mL/min; and nitenpyram analytical condition was 90% solvent A (50 mM KH 2PO 4, pH 2.5) and 10% solvent B (acetonitrile) at a flow rate of 1 mL/min. In results, retention times were 6.12, 8.63, 9.40 and 12.76 min for isoproturon, phenmedipham, pyridate and nitenpyram, respectively. All injection volumes were 10 μL and the limit of quantitation was 0.05 mg/kg for four pesticide compounds, respectively. Recovery rate test was performed with three farm products, rice, apple and soybean. Four pesticide compounds were spiked at concentrations of 0.05, 0.1 and 0.5 mg/kg. The recovery rates were ranged from 70.18% to 118.08% and the standard deviations of all experiments were within 10%.

AB - A method for the determination of four pesticide compounds, urea (isoproturon), bis-carbamate (phenmedipham), thiocarbamate (pyridate) and vinyllidenediamine (nitenpyram) were examined and analyzed by HPLC with C-18 column (250 mm×4.6 mm, 5 μm diameter particle size). Mobile phase consisted of deionized water, acetonitrile and 50 mM KH2PO4 (pH 2.5). Isoproturon and phenmedipham analytical condition was isocratic elution of the column with 50% solvent A (acetonitrile) and 50% solvent B (deionized water); pyridate was 85% solvent A (acetonitrile) and 15% solvent B (deionized water) at a flow rate of 1 mL/min; and nitenpyram analytical condition was 90% solvent A (50 mM KH 2PO 4, pH 2.5) and 10% solvent B (acetonitrile) at a flow rate of 1 mL/min. In results, retention times were 6.12, 8.63, 9.40 and 12.76 min for isoproturon, phenmedipham, pyridate and nitenpyram, respectively. All injection volumes were 10 μL and the limit of quantitation was 0.05 mg/kg for four pesticide compounds, respectively. Recovery rate test was performed with three farm products, rice, apple and soybean. Four pesticide compounds were spiked at concentrations of 0.05, 0.1 and 0.5 mg/kg. The recovery rates were ranged from 70.18% to 118.08% and the standard deviations of all experiments were within 10%.

KW - Analysis

KW - HPLC

KW - Isoproturon

KW - Nitenpyram

KW - Pesticide

KW - Phenmedipham

KW - Pyridate

KW - Residue

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