Analysis of genes responding to ultraviolet B irradiation of HaCaT keratinocytes using a cDNA microarray

K. M. Lee, J. G. Lee, E. Y. Seo, W. H. Lee, Y. H. Nam, J. M. Yang, Sun-Ho Kee, Y. J. Seo, J. K. Park, C. D. Kim, Jeung Hoon Lee

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Background; Ultraviolet (UV) B irradiation causes many important biological changes in skin, which lead to pathophysiological alterations of the homeostatic environment. Objectives: To gain more insight into the molecular events provoked by UVB irradiation, we performed cDNA microarray analysis. Methods: Immortalized HaCaT keratinocytes were irradiated with a high cytotoxic dose of UVB (50 mJ cm-2), and total RNA was isolated. Fluorescently labelled probes were prepared by reverse transcription and were hybridized with cDNA microarray slides made using 840 cDNA clones. Results: Time-course cDNA microarray analysis revealed the global gene expression profile after UVB exposure. Of 840 genes tested, 192 genes showed changes in their expression levels at one or more of four time points. The genes were clustered into four groups according to their expression patterns in a self-organizing maps analysis. Classification of these genes into nine functional categories revealed that UVB irradiation affected several biological processes. The genes that were first upregulated and then returned to normal levels included several genes related to the inhibition of cell growth and the proteasome pathway. Conversely, the expressions of many genes involved in the cytoskeleton, signal transduction, metabolism and transcription were first downregulated or unchanged and then upregulated later, reflecting the recovery of UVB-damaged cellular activities. Conclusions: These results demonstrate the complexity of the transcriptional profile of the UVB response, and provide a basis for the global characterization of UV-regulated gene expression.

Original languageEnglish
Pages (from-to)52-59
Number of pages8
JournalBritish Journal of Dermatology
Volume152
Issue number1
DOIs
Publication statusPublished - 2005 Jan 1

Fingerprint

Oligonucleotide Array Sequence Analysis
Keratinocytes
Genes
Microarray Analysis
Biological Phenomena
Gene Expression
Proteasome Endopeptidase Complex
Cytoskeleton
Transcriptome
Reverse Transcription
Signal Transduction
Down-Regulation
Complementary DNA
Clone Cells
RNA
Skin
Growth

Keywords

  • cDNA microarray
  • HaCaT cells
  • Ultraviolet B

ASJC Scopus subject areas

  • Dermatology

Cite this

Analysis of genes responding to ultraviolet B irradiation of HaCaT keratinocytes using a cDNA microarray. / Lee, K. M.; Lee, J. G.; Seo, E. Y.; Lee, W. H.; Nam, Y. H.; Yang, J. M.; Kee, Sun-Ho; Seo, Y. J.; Park, J. K.; Kim, C. D.; Lee, Jeung Hoon.

In: British Journal of Dermatology, Vol. 152, No. 1, 01.01.2005, p. 52-59.

Research output: Contribution to journalArticle

Lee, KM, Lee, JG, Seo, EY, Lee, WH, Nam, YH, Yang, JM, Kee, S-H, Seo, YJ, Park, JK, Kim, CD & Lee, JH 2005, 'Analysis of genes responding to ultraviolet B irradiation of HaCaT keratinocytes using a cDNA microarray', British Journal of Dermatology, vol. 152, no. 1, pp. 52-59. https://doi.org/10.1111/j.1365-2133.2005.06412.x
Lee, K. M. ; Lee, J. G. ; Seo, E. Y. ; Lee, W. H. ; Nam, Y. H. ; Yang, J. M. ; Kee, Sun-Ho ; Seo, Y. J. ; Park, J. K. ; Kim, C. D. ; Lee, Jeung Hoon. / Analysis of genes responding to ultraviolet B irradiation of HaCaT keratinocytes using a cDNA microarray. In: British Journal of Dermatology. 2005 ; Vol. 152, No. 1. pp. 52-59.
@article{5bdaea899fa84d0ea85c3d4d60ccd244,
title = "Analysis of genes responding to ultraviolet B irradiation of HaCaT keratinocytes using a cDNA microarray",
abstract = "Background; Ultraviolet (UV) B irradiation causes many important biological changes in skin, which lead to pathophysiological alterations of the homeostatic environment. Objectives: To gain more insight into the molecular events provoked by UVB irradiation, we performed cDNA microarray analysis. Methods: Immortalized HaCaT keratinocytes were irradiated with a high cytotoxic dose of UVB (50 mJ cm-2), and total RNA was isolated. Fluorescently labelled probes were prepared by reverse transcription and were hybridized with cDNA microarray slides made using 840 cDNA clones. Results: Time-course cDNA microarray analysis revealed the global gene expression profile after UVB exposure. Of 840 genes tested, 192 genes showed changes in their expression levels at one or more of four time points. The genes were clustered into four groups according to their expression patterns in a self-organizing maps analysis. Classification of these genes into nine functional categories revealed that UVB irradiation affected several biological processes. The genes that were first upregulated and then returned to normal levels included several genes related to the inhibition of cell growth and the proteasome pathway. Conversely, the expressions of many genes involved in the cytoskeleton, signal transduction, metabolism and transcription were first downregulated or unchanged and then upregulated later, reflecting the recovery of UVB-damaged cellular activities. Conclusions: These results demonstrate the complexity of the transcriptional profile of the UVB response, and provide a basis for the global characterization of UV-regulated gene expression.",
keywords = "cDNA microarray, HaCaT cells, Ultraviolet B",
author = "Lee, {K. M.} and Lee, {J. G.} and Seo, {E. Y.} and Lee, {W. H.} and Nam, {Y. H.} and Yang, {J. M.} and Sun-Ho Kee and Seo, {Y. J.} and Park, {J. K.} and Kim, {C. D.} and Lee, {Jeung Hoon}",
year = "2005",
month = "1",
day = "1",
doi = "10.1111/j.1365-2133.2005.06412.x",
language = "English",
volume = "152",
pages = "52--59",
journal = "British Journal of Dermatology",
issn = "0007-0963",
publisher = "Wiley-Blackwell",
number = "1",

}

TY - JOUR

T1 - Analysis of genes responding to ultraviolet B irradiation of HaCaT keratinocytes using a cDNA microarray

AU - Lee, K. M.

AU - Lee, J. G.

AU - Seo, E. Y.

AU - Lee, W. H.

AU - Nam, Y. H.

AU - Yang, J. M.

AU - Kee, Sun-Ho

AU - Seo, Y. J.

AU - Park, J. K.

AU - Kim, C. D.

AU - Lee, Jeung Hoon

PY - 2005/1/1

Y1 - 2005/1/1

N2 - Background; Ultraviolet (UV) B irradiation causes many important biological changes in skin, which lead to pathophysiological alterations of the homeostatic environment. Objectives: To gain more insight into the molecular events provoked by UVB irradiation, we performed cDNA microarray analysis. Methods: Immortalized HaCaT keratinocytes were irradiated with a high cytotoxic dose of UVB (50 mJ cm-2), and total RNA was isolated. Fluorescently labelled probes were prepared by reverse transcription and were hybridized with cDNA microarray slides made using 840 cDNA clones. Results: Time-course cDNA microarray analysis revealed the global gene expression profile after UVB exposure. Of 840 genes tested, 192 genes showed changes in their expression levels at one or more of four time points. The genes were clustered into four groups according to their expression patterns in a self-organizing maps analysis. Classification of these genes into nine functional categories revealed that UVB irradiation affected several biological processes. The genes that were first upregulated and then returned to normal levels included several genes related to the inhibition of cell growth and the proteasome pathway. Conversely, the expressions of many genes involved in the cytoskeleton, signal transduction, metabolism and transcription were first downregulated or unchanged and then upregulated later, reflecting the recovery of UVB-damaged cellular activities. Conclusions: These results demonstrate the complexity of the transcriptional profile of the UVB response, and provide a basis for the global characterization of UV-regulated gene expression.

AB - Background; Ultraviolet (UV) B irradiation causes many important biological changes in skin, which lead to pathophysiological alterations of the homeostatic environment. Objectives: To gain more insight into the molecular events provoked by UVB irradiation, we performed cDNA microarray analysis. Methods: Immortalized HaCaT keratinocytes were irradiated with a high cytotoxic dose of UVB (50 mJ cm-2), and total RNA was isolated. Fluorescently labelled probes were prepared by reverse transcription and were hybridized with cDNA microarray slides made using 840 cDNA clones. Results: Time-course cDNA microarray analysis revealed the global gene expression profile after UVB exposure. Of 840 genes tested, 192 genes showed changes in their expression levels at one or more of four time points. The genes were clustered into four groups according to their expression patterns in a self-organizing maps analysis. Classification of these genes into nine functional categories revealed that UVB irradiation affected several biological processes. The genes that were first upregulated and then returned to normal levels included several genes related to the inhibition of cell growth and the proteasome pathway. Conversely, the expressions of many genes involved in the cytoskeleton, signal transduction, metabolism and transcription were first downregulated or unchanged and then upregulated later, reflecting the recovery of UVB-damaged cellular activities. Conclusions: These results demonstrate the complexity of the transcriptional profile of the UVB response, and provide a basis for the global characterization of UV-regulated gene expression.

KW - cDNA microarray

KW - HaCaT cells

KW - Ultraviolet B

UR - http://www.scopus.com/inward/record.url?scp=19944430562&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=19944430562&partnerID=8YFLogxK

U2 - 10.1111/j.1365-2133.2005.06412.x

DO - 10.1111/j.1365-2133.2005.06412.x

M3 - Article

VL - 152

SP - 52

EP - 59

JO - British Journal of Dermatology

JF - British Journal of Dermatology

SN - 0007-0963

IS - 1

ER -