Analysis of neuron-like differentiation of human bone marrow mesenchymal stem cells

Chi Bong Choi, Yun Kyoung Cho, K. V. Bhanu Prakash, Bo Keun Jee, Chang Whan Han, Young Ki Paik, Hwi Yool Kim, Kweon Haeng Lee, Namhyun Chung, Hyoung Kyun Rha

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Abstract

The objective of the study was to evaluate differentiation of human bone marrow mesenchymal stem cells into true or pseudo neurons after treating with chemical induction medium in vitro. The morphological changes were assessed using interference contrast microscopy. Immunocytochemistry and Western blotting were performed using neuronal markers. Further evaluation was conducted with proteomic profiling, DNA microarray analysis and the whole-cell patch clamp test. After three hours of treatment with chemical induction medium, nearly three-fourths of the hMSCs changed to cells with a neuronal phenotype. The results of immunocytochemistry and Western blotting showed a high expression of neuronal markers in these cells at 3 h which decreased at 24 h. The proteomics analysis showed no change of proteins related to neuronal differentiation. DNA microarray showed downregulation of neuron related genes. The patch clamp test was unable to demonstrate any similarity to true neurons. Our findings suggest that neuron-like cells derived from chemical induction of hMSCs are not the genuine neurons as they resemble true neurons phenotypically but are different in genotypic and electrophysiological characteristics.

Original languageEnglish
Pages (from-to)138-146
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume350
Issue number1
DOIs
Publication statusPublished - 2006 Nov 10

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Keywords

  • Chemical induced medium
  • DNA microarray analysis
  • Human mesenchymal stem cells
  • Neuronal markers
  • Proteomic profiling
  • Whole-cell patch clamp test

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Choi, C. B., Cho, Y. K., Bhanu Prakash, K. V., Jee, B. K., Han, C. W., Paik, Y. K., Kim, H. Y., Lee, K. H., Chung, N., & Rha, H. K. (2006). Analysis of neuron-like differentiation of human bone marrow mesenchymal stem cells. Biochemical and Biophysical Research Communications, 350(1), 138-146. https://doi.org/10.1016/j.bbrc.2006.09.010