Analysis of passive mixing behavior in a poly(dimethylsiloxane) microfluidic channel using confocal fluorescence and Raman microscopy

Taehan Park, Moonkwon Lee, Jaebum Choo, Yang S. Kim, Eun Kyu Lee, Duck Joong Kim, Sang Hoon Lee

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Confocal fluorescence microscopy (CFM) and confocal Raman microscopy (CRM) have been applied to monitor the laminar flow mixing behavior in a poly(dimethylsiloxane) (PDMS) microfluidic channel. Two passive PDMS micromixing devices were fabricated for this purpose: a two-dimensional round-wave channel and a three-dimensional serpentine channel. The microscale laminar flow mixing of ethanol and isopropanol was evaluated using the CFM and CRM at various flow rates. The mixing behavior of confluent streams in the microchannel was assessed by determining the degree of color change in Rhodamine 6G dye on mixing using the CFM. However, it was also possible to quantitatively evaluate the mixing process without employing a fluorescence label using the CRM. The results show a strong potential for CRM as a highly sensitive detection tool to measure fundamental fluid mixing processes and to provide detailed information on chemical changes of non-fluorescent reaction mixtures in a PDMS microfluidic channel.

Original languageEnglish
Pages (from-to)1172-1179
Number of pages8
JournalApplied Spectroscopy
Volume58
Issue number10
DOIs
Publication statusPublished - 2004 Oct 1
Externally publishedYes

Fingerprint

Microfluidics
Polydimethylsiloxane
Fluorescence Microscopy
Confocal Microscopy
Microscopic examination
Fluorescence
microscopy
fluorescence
Confocal microscopy
Fluorescence microscopy
Laminar flow
laminar flow
2-Propanol
Microchannels
rhodamine
microchannels
baysilon
Labels
microbalances
Ethanol

Keywords

  • Confocal fluorescence microscopy
  • Confocal Raman microscopy
  • Laminar flow mixing
  • Microfluidic channel
  • PDMS
  • Poly(dimethylsiloxane)

ASJC Scopus subject areas

  • Spectroscopy
  • Instrumentation

Cite this

Analysis of passive mixing behavior in a poly(dimethylsiloxane) microfluidic channel using confocal fluorescence and Raman microscopy. / Park, Taehan; Lee, Moonkwon; Choo, Jaebum; Kim, Yang S.; Lee, Eun Kyu; Kim, Duck Joong; Lee, Sang Hoon.

In: Applied Spectroscopy, Vol. 58, No. 10, 01.10.2004, p. 1172-1179.

Research output: Contribution to journalArticle

Park, Taehan ; Lee, Moonkwon ; Choo, Jaebum ; Kim, Yang S. ; Lee, Eun Kyu ; Kim, Duck Joong ; Lee, Sang Hoon. / Analysis of passive mixing behavior in a poly(dimethylsiloxane) microfluidic channel using confocal fluorescence and Raman microscopy. In: Applied Spectroscopy. 2004 ; Vol. 58, No. 10. pp. 1172-1179.
@article{051f0cbcaccc4448a3ec07ca655472ff,
title = "Analysis of passive mixing behavior in a poly(dimethylsiloxane) microfluidic channel using confocal fluorescence and Raman microscopy",
abstract = "Confocal fluorescence microscopy (CFM) and confocal Raman microscopy (CRM) have been applied to monitor the laminar flow mixing behavior in a poly(dimethylsiloxane) (PDMS) microfluidic channel. Two passive PDMS micromixing devices were fabricated for this purpose: a two-dimensional round-wave channel and a three-dimensional serpentine channel. The microscale laminar flow mixing of ethanol and isopropanol was evaluated using the CFM and CRM at various flow rates. The mixing behavior of confluent streams in the microchannel was assessed by determining the degree of color change in Rhodamine 6G dye on mixing using the CFM. However, it was also possible to quantitatively evaluate the mixing process without employing a fluorescence label using the CRM. The results show a strong potential for CRM as a highly sensitive detection tool to measure fundamental fluid mixing processes and to provide detailed information on chemical changes of non-fluorescent reaction mixtures in a PDMS microfluidic channel.",
keywords = "Confocal fluorescence microscopy, Confocal Raman microscopy, Laminar flow mixing, Microfluidic channel, PDMS, Poly(dimethylsiloxane)",
author = "Taehan Park and Moonkwon Lee and Jaebum Choo and Kim, {Yang S.} and Lee, {Eun Kyu} and Kim, {Duck Joong} and Lee, {Sang Hoon}",
year = "2004",
month = "10",
day = "1",
doi = "10.1366/0003702042336019",
language = "English",
volume = "58",
pages = "1172--1179",
journal = "Applied Spectroscopy",
issn = "0003-7028",
publisher = "Society for Applied Spectroscopy",
number = "10",

}

TY - JOUR

T1 - Analysis of passive mixing behavior in a poly(dimethylsiloxane) microfluidic channel using confocal fluorescence and Raman microscopy

AU - Park, Taehan

AU - Lee, Moonkwon

AU - Choo, Jaebum

AU - Kim, Yang S.

AU - Lee, Eun Kyu

AU - Kim, Duck Joong

AU - Lee, Sang Hoon

PY - 2004/10/1

Y1 - 2004/10/1

N2 - Confocal fluorescence microscopy (CFM) and confocal Raman microscopy (CRM) have been applied to monitor the laminar flow mixing behavior in a poly(dimethylsiloxane) (PDMS) microfluidic channel. Two passive PDMS micromixing devices were fabricated for this purpose: a two-dimensional round-wave channel and a three-dimensional serpentine channel. The microscale laminar flow mixing of ethanol and isopropanol was evaluated using the CFM and CRM at various flow rates. The mixing behavior of confluent streams in the microchannel was assessed by determining the degree of color change in Rhodamine 6G dye on mixing using the CFM. However, it was also possible to quantitatively evaluate the mixing process without employing a fluorescence label using the CRM. The results show a strong potential for CRM as a highly sensitive detection tool to measure fundamental fluid mixing processes and to provide detailed information on chemical changes of non-fluorescent reaction mixtures in a PDMS microfluidic channel.

AB - Confocal fluorescence microscopy (CFM) and confocal Raman microscopy (CRM) have been applied to monitor the laminar flow mixing behavior in a poly(dimethylsiloxane) (PDMS) microfluidic channel. Two passive PDMS micromixing devices were fabricated for this purpose: a two-dimensional round-wave channel and a three-dimensional serpentine channel. The microscale laminar flow mixing of ethanol and isopropanol was evaluated using the CFM and CRM at various flow rates. The mixing behavior of confluent streams in the microchannel was assessed by determining the degree of color change in Rhodamine 6G dye on mixing using the CFM. However, it was also possible to quantitatively evaluate the mixing process without employing a fluorescence label using the CRM. The results show a strong potential for CRM as a highly sensitive detection tool to measure fundamental fluid mixing processes and to provide detailed information on chemical changes of non-fluorescent reaction mixtures in a PDMS microfluidic channel.

KW - Confocal fluorescence microscopy

KW - Confocal Raman microscopy

KW - Laminar flow mixing

KW - Microfluidic channel

KW - PDMS

KW - Poly(dimethylsiloxane)

UR - http://www.scopus.com/inward/record.url?scp=8344271013&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=8344271013&partnerID=8YFLogxK

U2 - 10.1366/0003702042336019

DO - 10.1366/0003702042336019

M3 - Article

VL - 58

SP - 1172

EP - 1179

JO - Applied Spectroscopy

JF - Applied Spectroscopy

SN - 0003-7028

IS - 10

ER -