Analysis of passive mixing behavior in a poly(dimethylsiloxane) microfluidic channel using confocal fluorescence and Raman microscopy

Taehan Park, Moonkwon Lee, Jaebum Choo, Yang S. Kim, Eun Kyu Lee, Duck Joong Kim, Sang Hoon Lee

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Confocal fluorescence microscopy (CFM) and confocal Raman microscopy (CRM) have been applied to monitor the laminar flow mixing behavior in a poly(dimethylsiloxane) (PDMS) microfluidic channel. Two passive PDMS micromixing devices were fabricated for this purpose: a two-dimensional round-wave channel and a three-dimensional serpentine channel. The microscale laminar flow mixing of ethanol and isopropanol was evaluated using the CFM and CRM at various flow rates. The mixing behavior of confluent streams in the microchannel was assessed by determining the degree of color change in Rhodamine 6G dye on mixing using the CFM. However, it was also possible to quantitatively evaluate the mixing process without employing a fluorescence label using the CRM. The results show a strong potential for CRM as a highly sensitive detection tool to measure fundamental fluid mixing processes and to provide detailed information on chemical changes of non-fluorescent reaction mixtures in a PDMS microfluidic channel.

Original languageEnglish
Pages (from-to)1172-1179
Number of pages8
JournalApplied Spectroscopy
Volume58
Issue number10
DOIs
Publication statusPublished - 2004 Oct

Keywords

  • Confocal Raman microscopy
  • Confocal fluorescence microscopy
  • Laminar flow mixing
  • Microfluidic channel
  • PDMS
  • Poly(dimethylsiloxane)

ASJC Scopus subject areas

  • Instrumentation
  • Spectroscopy

Fingerprint Dive into the research topics of 'Analysis of passive mixing behavior in a poly(dimethylsiloxane) microfluidic channel using confocal fluorescence and Raman microscopy'. Together they form a unique fingerprint.

  • Cite this