Analysis of Plasmodium vivax merozoite surface protein-1 gene sequences from resurgent Korean isolates

Chae Seung Lim, Sang Hyun Kim, So I. Kwon, Jin Won Song, Ki-Joon Song, Kap N. Lee

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

The merozoite surface protein-1 (MSP-1) of Plasmodium vivax exhibits great antigenic diversity among different isolates of this parasite. This antigen is a useful genetic marker for studying the polymorphism of natural P. vivax parasite populations. One or more of these populations has been responsible for resurgent malaria now occurring in Korea. This paper reports the analysis of a highly polymorphic region between interspecies conserved blocks 5 and 6 of the MSP-1 gene, using the polymerase chain reaction to amplify the DNA fragment encompassing these regions from 25 Korean isolates, followed by sequencing. Almost all amino acid sequences of Korean isolates were nearly identical to that of Thai isolates TD525A (96.6-99.7%) and TD424 (96.3-99.5%), and very similar to that of the France-Belem strain when compared with other isolates (Sal-1, Sri Lanka, and Colombia). Interallelic recombination was found in the poly-Q repeat and a Sal-1 type amino acid structure was observed in all isolates. This study shows that the MSP gene nucleotide sequence of resurgent P. vivax in Korea is most similar to that of Thai isolates; however, the Korean strains are phylogenetically unique.

Original languageEnglish
Pages (from-to)261-265
Number of pages5
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume62
Issue number2
Publication statusPublished - 2000 Feb 1

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Merozoite Surface Protein 1
Plasmodium vivax
Korea
Parasites
Genes
Antigenic Variation
Sri Lanka
Colombia
Genetic Markers
Genetic Recombination
Population
Malaria
France
Amino Acid Sequence
Antigens
Amino Acids
Polymerase Chain Reaction
DNA

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Analysis of Plasmodium vivax merozoite surface protein-1 gene sequences from resurgent Korean isolates. / Lim, Chae Seung; Kim, Sang Hyun; Kwon, So I.; Song, Jin Won; Song, Ki-Joon; Lee, Kap N.

In: American Journal of Tropical Medicine and Hygiene, Vol. 62, No. 2, 01.02.2000, p. 261-265.

Research output: Contribution to journalArticle

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