Analysis of protein redox modification by hypoxia

Kyoung Soo Choi, Soo Yeon Park, Sun Hee Baek, Rama Dey-Rao, Young Mee Park, Haitao Zhang, Clement Ip, Eun Mi Park, Yeul Hong Kim, Hoon Park Jong

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

We examined hypoxia-induced changes in global thiol proteome profile in human prostate cancer cells using a BIAM-based display method. We analyzed the kinetics of protein thiol modification by using a pattern recognition algorithm, self-organizing maps (SOM) clustering, and identified the BIAM-labeled proteins by MALDI-TOF and ESI-tandem mass spectrometry. We found 99 out of 215 of total BIAM-labeled proteins were affected by hypoxia treatment and, yet, with diverse patterns and kinetics of redox modification. Our study proved that proteomics analysis employing the BIAM-labeling method can provide valuable information pertaining to global changes in the redox status of proteins in response to hypoxia.

Original languageEnglish
Pages (from-to)65-79
Number of pages15
JournalPreparative Biochemistry and Biotechnology
Volume36
Issue number1
DOIs
Publication statusPublished - 2006 Feb 1

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Oxidation-Reduction
Sulfhydryl Compounds
Proteins
Kinetics
Electrospray Ionization Mass Spectrometry
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Self organizing maps
Proteome
Tandem Mass Spectrometry
Proteomics
Labeling
Pattern recognition
Mass spectrometry
Cluster Analysis
Prostatic Neoplasms
Display devices
Cells
Hypoxia

Keywords

  • Hypoxia
  • Oxidative stress
  • Proteomics
  • Redox modification

ASJC Scopus subject areas

  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Biotechnology

Cite this

Choi, K. S., Park, S. Y., Baek, S. H., Dey-Rao, R., Park, Y. M., Zhang, H., ... Jong, H. P. (2006). Analysis of protein redox modification by hypoxia. Preparative Biochemistry and Biotechnology, 36(1), 65-79. https://doi.org/10.1080/10826060500388520

Analysis of protein redox modification by hypoxia. / Choi, Kyoung Soo; Park, Soo Yeon; Baek, Sun Hee; Dey-Rao, Rama; Park, Young Mee; Zhang, Haitao; Ip, Clement; Park, Eun Mi; Kim, Yeul Hong; Jong, Hoon Park.

In: Preparative Biochemistry and Biotechnology, Vol. 36, No. 1, 01.02.2006, p. 65-79.

Research output: Contribution to journalArticle

Choi, KS, Park, SY, Baek, SH, Dey-Rao, R, Park, YM, Zhang, H, Ip, C, Park, EM, Kim, YH & Jong, HP 2006, 'Analysis of protein redox modification by hypoxia', Preparative Biochemistry and Biotechnology, vol. 36, no. 1, pp. 65-79. https://doi.org/10.1080/10826060500388520
Choi KS, Park SY, Baek SH, Dey-Rao R, Park YM, Zhang H et al. Analysis of protein redox modification by hypoxia. Preparative Biochemistry and Biotechnology. 2006 Feb 1;36(1):65-79. https://doi.org/10.1080/10826060500388520
Choi, Kyoung Soo ; Park, Soo Yeon ; Baek, Sun Hee ; Dey-Rao, Rama ; Park, Young Mee ; Zhang, Haitao ; Ip, Clement ; Park, Eun Mi ; Kim, Yeul Hong ; Jong, Hoon Park. / Analysis of protein redox modification by hypoxia. In: Preparative Biochemistry and Biotechnology. 2006 ; Vol. 36, No. 1. pp. 65-79.
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