Analysis of protein redox modification by hypoxia

Kyoung Soo Choi, Soo Yeon Park, Sun Hee Baek, Rama Dey-Rao, Young Mee Park, Haitao Zhang, Clement Ip, Eun Mi Park, Hong Kim Yeul, Hoon Park Jong

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

We examined hypoxia-induced changes in global thiol proteome profile in human prostate cancer cells using a BIAM-based display method. We analyzed the kinetics of protein thiol modification by using a pattern recognition algorithm, self-organizing maps (SOM) clustering, and identified the BIAM-labeled proteins by MALDI-TOF and ESI-tandem mass spectrometry. We found 99 out of 215 of total BIAM-labeled proteins were affected by hypoxia treatment and, yet, with diverse patterns and kinetics of redox modification. Our study proved that proteomics analysis employing the BIAM-labeling method can provide valuable information pertaining to global changes in the redox status of proteins in response to hypoxia.

Original languageEnglish
Pages (from-to)65-79
Number of pages15
JournalPreparative Biochemistry and Biotechnology
Volume36
Issue number1
DOIs
Publication statusPublished - 2006 Feb 1

Keywords

  • Hypoxia
  • Oxidative stress
  • Proteomics
  • Redox modification

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry

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    Choi, K. S., Park, S. Y., Baek, S. H., Dey-Rao, R., Park, Y. M., Zhang, H., Ip, C., Park, E. M., Yeul, H. K., & Jong, H. P. (2006). Analysis of protein redox modification by hypoxia. Preparative Biochemistry and Biotechnology, 36(1), 65-79. https://doi.org/10.1080/10826060500388520