Analysis of proteins expressed in rat plasma exposed to dioxin using 2-dimensional gel electrophoresis

Won Kyu Son, Do Youn Lee, Sung Han Lee, Won A. Joo, Chan Wha Kim

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Dioxins are a class of polyhalogenated aromatic hydrocarbons that induces a wide spectrum of toxic responses in animals. In this study, two groups of Sprague-Dawley rats were exposed to 2,3,7,8-tetrachlorobenzo-p-dioxin (TCDD); one group received short-term exposure at a single dose of 1, 10, 20 or 50 μg/kg body weight and the other received long-term exposure to a daily low dose of 0.01, 0.1, 1 or 2.5 μg/kg body weight for one month. Two-dimensional electrophoresis was utilized to resolve the protein profile of rat plasma exposed to TCDD at different doses. One novel and three volume-increased spots were identified and characterized by matrix-assisted laser desorption/ionization-time of flight mass spectrometry and electrospray-ionization on quadropole-TOF2 mass spectrometry. The novel protein was identified as plasma glutathione peroxidase precursor and the volume-increased proteins were cytokeratin 8 polypeptide, Ig lambda-1 chain C region and Ig lambda-2 chain C region. These proteins may be used as biomarkers to diagnose dioxin exposure and may help in understanding the toxic effects of dioxins.

Original languageEnglish
Pages (from-to)2393-2401
Number of pages9
JournalProteomics
Volume3
Issue number12
DOIs
Publication statusPublished - 2003 Dec 1

Keywords

  • 2,3,7,8-Tetra-chlorobenzo-p-dioxin
  • Electrospray ionization-quadrupole time of flight tandem mass spectrometry
  • Matrix-assisted laser desorption/ionization-time of flight mass spectrometry
  • Rat plasma
  • Two-dimensional gel electrophoresis

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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