Analysis of proteins expressed in rat plasma exposed to dioxin using 2-dimensional gel electrophoresis

Won Kyu Son, Do Youn Lee, Sung Han Lee, Won A. Joo, Chan Wha Kim

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Dioxins are a class of polyhalogenated aromatic hydrocarbons that induces a wide spectrum of toxic responses in animals. In this study, two groups of Sprague-Dawley rats were exposed to 2,3,7,8-tetrachlorobenzo-p-dioxin (TCDD); one group received short-term exposure at a single dose of 1, 10, 20 or 50 μg/kg body weight and the other received long-term exposure to a daily low dose of 0.01, 0.1, 1 or 2.5 μg/kg body weight for one month. Two-dimensional electrophoresis was utilized to resolve the protein profile of rat plasma exposed to TCDD at different doses. One novel and three volume-increased spots were identified and characterized by matrix-assisted laser desorption/ionization-time of flight mass spectrometry and electrospray-ionization on quadropole-TOF2 mass spectrometry. The novel protein was identified as plasma glutathione peroxidase precursor and the volume-increased proteins were cytokeratin 8 polypeptide, Ig lambda-1 chain C region and Ig lambda-2 chain C region. These proteins may be used as biomarkers to diagnose dioxin exposure and may help in understanding the toxic effects of dioxins.

Original languageEnglish
Pages (from-to)2393-2401
Number of pages9
JournalProteomics
Volume3
Issue number12
DOIs
Publication statusPublished - 2003 Dec 1

Fingerprint

Dioxins
Electrophoresis
Rats
Gels
Plasmas
Poisons
Mass spectrometry
Proteins
Body Weight
Keratin-8
Aromatic Hydrocarbons
Electrospray ionization
Electrospray Ionization Mass Spectrometry
Biomarkers
Glutathione Peroxidase
Ionization
Sprague Dawley Rats
Desorption
Mass Spectrometry
Animals

Keywords

  • 2,3,7,8-Tetra-chlorobenzo-p-dioxin
  • Electrospray ionization-quadrupole time of flight tandem mass spectrometry
  • Matrix-assisted laser desorption/ionization-time of flight mass spectrometry
  • Rat plasma
  • Two-dimensional gel electrophoresis

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Analysis of proteins expressed in rat plasma exposed to dioxin using 2-dimensional gel electrophoresis. / Son, Won Kyu; Lee, Do Youn; Lee, Sung Han; Joo, Won A.; Kim, Chan Wha.

In: Proteomics, Vol. 3, No. 12, 01.12.2003, p. 2393-2401.

Research output: Contribution to journalArticle

Son, Won Kyu ; Lee, Do Youn ; Lee, Sung Han ; Joo, Won A. ; Kim, Chan Wha. / Analysis of proteins expressed in rat plasma exposed to dioxin using 2-dimensional gel electrophoresis. In: Proteomics. 2003 ; Vol. 3, No. 12. pp. 2393-2401.
@article{2a97661e42b6499fa70fbbe921a4e2d5,
title = "Analysis of proteins expressed in rat plasma exposed to dioxin using 2-dimensional gel electrophoresis",
abstract = "Dioxins are a class of polyhalogenated aromatic hydrocarbons that induces a wide spectrum of toxic responses in animals. In this study, two groups of Sprague-Dawley rats were exposed to 2,3,7,8-tetrachlorobenzo-p-dioxin (TCDD); one group received short-term exposure at a single dose of 1, 10, 20 or 50 μg/kg body weight and the other received long-term exposure to a daily low dose of 0.01, 0.1, 1 or 2.5 μg/kg body weight for one month. Two-dimensional electrophoresis was utilized to resolve the protein profile of rat plasma exposed to TCDD at different doses. One novel and three volume-increased spots were identified and characterized by matrix-assisted laser desorption/ionization-time of flight mass spectrometry and electrospray-ionization on quadropole-TOF2 mass spectrometry. The novel protein was identified as plasma glutathione peroxidase precursor and the volume-increased proteins were cytokeratin 8 polypeptide, Ig lambda-1 chain C region and Ig lambda-2 chain C region. These proteins may be used as biomarkers to diagnose dioxin exposure and may help in understanding the toxic effects of dioxins.",
keywords = "2,3,7,8-Tetra-chlorobenzo-p-dioxin, Electrospray ionization-quadrupole time of flight tandem mass spectrometry, Matrix-assisted laser desorption/ionization-time of flight mass spectrometry, Rat plasma, Two-dimensional gel electrophoresis",
author = "Son, {Won Kyu} and Lee, {Do Youn} and Lee, {Sung Han} and Joo, {Won A.} and Kim, {Chan Wha}",
year = "2003",
month = "12",
day = "1",
doi = "10.1002/pmic.200300605",
language = "English",
volume = "3",
pages = "2393--2401",
journal = "Proteomics",
issn = "1615-9853",
publisher = "Wiley-VCH Verlag",
number = "12",

}

TY - JOUR

T1 - Analysis of proteins expressed in rat plasma exposed to dioxin using 2-dimensional gel electrophoresis

AU - Son, Won Kyu

AU - Lee, Do Youn

AU - Lee, Sung Han

AU - Joo, Won A.

AU - Kim, Chan Wha

PY - 2003/12/1

Y1 - 2003/12/1

N2 - Dioxins are a class of polyhalogenated aromatic hydrocarbons that induces a wide spectrum of toxic responses in animals. In this study, two groups of Sprague-Dawley rats were exposed to 2,3,7,8-tetrachlorobenzo-p-dioxin (TCDD); one group received short-term exposure at a single dose of 1, 10, 20 or 50 μg/kg body weight and the other received long-term exposure to a daily low dose of 0.01, 0.1, 1 or 2.5 μg/kg body weight for one month. Two-dimensional electrophoresis was utilized to resolve the protein profile of rat plasma exposed to TCDD at different doses. One novel and three volume-increased spots were identified and characterized by matrix-assisted laser desorption/ionization-time of flight mass spectrometry and electrospray-ionization on quadropole-TOF2 mass spectrometry. The novel protein was identified as plasma glutathione peroxidase precursor and the volume-increased proteins were cytokeratin 8 polypeptide, Ig lambda-1 chain C region and Ig lambda-2 chain C region. These proteins may be used as biomarkers to diagnose dioxin exposure and may help in understanding the toxic effects of dioxins.

AB - Dioxins are a class of polyhalogenated aromatic hydrocarbons that induces a wide spectrum of toxic responses in animals. In this study, two groups of Sprague-Dawley rats were exposed to 2,3,7,8-tetrachlorobenzo-p-dioxin (TCDD); one group received short-term exposure at a single dose of 1, 10, 20 or 50 μg/kg body weight and the other received long-term exposure to a daily low dose of 0.01, 0.1, 1 or 2.5 μg/kg body weight for one month. Two-dimensional electrophoresis was utilized to resolve the protein profile of rat plasma exposed to TCDD at different doses. One novel and three volume-increased spots were identified and characterized by matrix-assisted laser desorption/ionization-time of flight mass spectrometry and electrospray-ionization on quadropole-TOF2 mass spectrometry. The novel protein was identified as plasma glutathione peroxidase precursor and the volume-increased proteins were cytokeratin 8 polypeptide, Ig lambda-1 chain C region and Ig lambda-2 chain C region. These proteins may be used as biomarkers to diagnose dioxin exposure and may help in understanding the toxic effects of dioxins.

KW - 2,3,7,8-Tetra-chlorobenzo-p-dioxin

KW - Electrospray ionization-quadrupole time of flight tandem mass spectrometry

KW - Matrix-assisted laser desorption/ionization-time of flight mass spectrometry

KW - Rat plasma

KW - Two-dimensional gel electrophoresis

UR - http://www.scopus.com/inward/record.url?scp=0346887179&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0346887179&partnerID=8YFLogxK

U2 - 10.1002/pmic.200300605

DO - 10.1002/pmic.200300605

M3 - Article

C2 - 14673789

AN - SCOPUS:0346887179

VL - 3

SP - 2393

EP - 2401

JO - Proteomics

JF - Proteomics

SN - 1615-9853

IS - 12

ER -