Analysis of proteoglycan gene messages in human nasal mucosa and nasal polyp using dot blot hybridization

Sang Hag Lee, Ji Hoon Park, Byung Hoon Oh, Kwang-Yoon Jung, Heung Man Lee, Jong Ouck Choi, Hyun Ho Lim

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

It has been suggested that the formation and growth of nasal polyp require the remodeling of extracellular matrix. Proteoglycans (PGs) are major components of the extracellular matrix that maintain the integrity of structural tissue. The leucine-rich repeat PGs include lumican, decorin and biglycan and have many important biologic activities in various pathologic conditions, including the remodeling of the extracellular matrix. Therefore, these small-PG families may be involved in the formation and growth of nasal polyp. In the present study, surgical specimens of nasal polyps and nasal mucosa were assessed for expression of mRNA coding for lumican, decorin and biglycan using reverse transcriptase-polymerase chain reaction followed by dot blot hybridization. Lumican, decorin and biglycan mRNA were expressed in all tissue samples examined. Semiquantitative dot blot hybridization revealed that the levels of the lumican and biglycan messages are lower in nasal polyp tissues than in nasal mucosa. The decorin messages in nasal polyp were expressed at levels similar to those in nasal mucosa. These results suggest that lumican, decorin and biglycan may be important components of the extracellular matrix in nasal mucosa. Considering the function of these PGs, normal levels of decorin associated with low levels of biglycan and lumican may play a role in the pathogenesis of nasal polyposis.

Original languageEnglish
Pages (from-to)398-402
Number of pages5
JournalActa Oto-Laryngologica
Volume121
Issue number3
DOIs
Publication statusPublished - 2001 Jun 5

Fingerprint

Biglycan
Decorin
Nasal Polyps
Nasal Mucosa
Proteoglycans
Extracellular Matrix
Genes
Messenger RNA
Growth
Reverse Transcriptase Polymerase Chain Reaction
Nose
Leucine
Lumican

Keywords

  • Biglycan
  • Decorin
  • Lumican
  • Nasal mucosa
  • Nasal polyp

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Analysis of proteoglycan gene messages in human nasal mucosa and nasal polyp using dot blot hybridization. / Lee, Sang Hag; Park, Ji Hoon; Oh, Byung Hoon; Jung, Kwang-Yoon; Lee, Heung Man; Choi, Jong Ouck; Lim, Hyun Ho.

In: Acta Oto-Laryngologica, Vol. 121, No. 3, 05.06.2001, p. 398-402.

Research output: Contribution to journalArticle

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