Analysis of SiO2 nanoparticles binding proteins in rat blood and brain homogenate

Kyu Hwan Shim, John Hulme, Eun Ho Maeng, Meyoung-Kon Kim, Seong Soo A. An

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

A multitude of nanoparticles, such as titanium oxide (TiO2), zinc oxide, aluminum oxide, gold oxide, silver oxide, iron oxide, and silica oxide, are found in many chemical, cosmetic, pharmaceutical, and electronic products. Recently, SiO2 nanoparticles were shown to have an inert toxicity profile and no association with an irreversible toxicological change in animal models. Hence, exposure to SiO2 nanoparticles is on the increase. SiO2 nanoparticles are routinely used in numerous materials, from strengthening filler for concrete and other construction composites, to nontoxic platforms for biomedical application, such as drug delivery and theragnostics. On the other hand, recent in vitro experiments indicated that SiO2 nanoparticles were cytotoxic. Therefore, we investigated these nanoparticles to identify potentially toxic pathways by analyz­ing the adsorbed protein corona on the surface of SiO2 nanoparticles in the blood and brain of the rat. Four types of SiO2 nanoparticles were chosen for investigation, and the protein corona of each type was analyzed using liquid chromatography-tandem mass spectrometry technology. In total, 115 and 48 plasma proteins from the rat were identified as being bound to negatively charged 20 nm and 100 nm SiO2 nanoparticles, respectively, and 50 and 36 proteins were found for 20 nm and 100 nm arginine-coated SiO2 nanoparticles, respectively. Higher numbers of proteins were adsorbed onto the 20 nm sized SiO2 nanoparticles than onto the 100 nm sized nanoparticles regardless of charge. When proteins were compared between the two charges, higher numbers of proteins were found for arginine-coated positively charged SiO2 nanopar­ticles than for the negatively charged nanoparticles. The proteins identified as bound in the corona from SiO2 nanoparticles were further analyzed with ClueGO, a Cytoscape plugin used in protein ontology and for identifying biological interaction pathways. Proteins bound on the surface of nanoparticles may affect functional and conformational properties and distributions in complicated biological processes.

Original languageEnglish
Pages (from-to)207-215
Number of pages9
JournalInternational Journal of Nanomedicine
Volume9
DOIs
Publication statusPublished - 2014 Dec 15

Fingerprint

Nanoparticles
Rats
Brain
Carrier Proteins
Blood
Proteins
Arginine
Oxides
Biological Ontologies
Silver oxides
Zinc Oxide
Biological Phenomena
Cosmetics
Aluminum Oxide
Poisons
Titanium oxides
Liquid chromatography
Strengthening (metal)
Tandem Mass Spectrometry
Zinc oxide

Keywords

  • Brain homogenate
  • Nanoparticles
  • Nanotoxicity
  • Plasma
  • Protein corona
  • Silica

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Drug Discovery
  • Organic Chemistry

Cite this

Analysis of SiO2 nanoparticles binding proteins in rat blood and brain homogenate. / Shim, Kyu Hwan; Hulme, John; Maeng, Eun Ho; Kim, Meyoung-Kon; An, Seong Soo A.

In: International Journal of Nanomedicine, Vol. 9, 15.12.2014, p. 207-215.

Research output: Contribution to journalArticle

Shim, Kyu Hwan ; Hulme, John ; Maeng, Eun Ho ; Kim, Meyoung-Kon ; An, Seong Soo A. / Analysis of SiO2 nanoparticles binding proteins in rat blood and brain homogenate. In: International Journal of Nanomedicine. 2014 ; Vol. 9. pp. 207-215.
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