Abstract
Steroid hormones modulate a variety of physiological functions in the hypothalamus. We attempted to identify steroid-regulated genes in the rat preoptic area-anterior hypothalamus by comparing differentially expressed mRNAs. Adult female rats were ovariectomized and, 1 week later, a silastic capsule containing 17 β-oestradiol (180 μg/ml) was subcutaneously implanted. After 2 days, a single injection of progesterone (1 mg) was administered at 10.00 h and rats were killed at 17.00 h on the same day. Differential-display polymerase chain reaction followed by Northern blot analysis showed that 10 clones were differentially regulated. Using homology search in Genbank, three genes were identified as sodium, potassium-ATPase β1, protein kinase C-binding Nell-homologue protein and evectin-1. Further characterization of 10 clones showed that the expression patterns were tissue-specific and differentially regulated during puberty. Among these, mRNAs for protein kinase C-binding Nell-homologue protein, evectin-1 and human CGI-118 protein-like gene were induced after vagina opening, and differentially expressed during the oestrous cycle. Taken together, several steroid-regulated genes identified in the present study may play an important role in regulating hypothalamic functions, including puberty and the oestrous cycle.
| Original language | English |
|---|---|
| Pages (from-to) | 531-539 |
| Number of pages | 9 |
| Journal | Journal of Neuroendocrinology |
| Volume | 13 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 2001 Jun 18 |
| Externally published | Yes |
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Keywords
- DD-PCR
- Genes
- Oestrous cycle
- POA-AH
- Puberty
- Steroids
ASJC Scopus subject areas
- Endocrinology
- Neuroscience(all)
Cite this
Analysis of steroid-induced genes in the rat preoptic area-anterior hypothalamus using a differential-display reverse transcriptase-polymerase chain reaction. / Park, S.; Seong, Jae Young; Son, Gi Hoon; Kang, S. S.; Lee, S.; Kim, S. R.; Kim, K.
In: Journal of Neuroendocrinology, Vol. 13, No. 6, 18.06.2001, p. 531-539.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Analysis of steroid-induced genes in the rat preoptic area-anterior hypothalamus using a differential-display reverse transcriptase-polymerase chain reaction
AU - Park, S.
AU - Seong, Jae Young
AU - Son, Gi Hoon
AU - Kang, S. S.
AU - Lee, S.
AU - Kim, S. R.
AU - Kim, K.
PY - 2001/6/18
Y1 - 2001/6/18
N2 - Steroid hormones modulate a variety of physiological functions in the hypothalamus. We attempted to identify steroid-regulated genes in the rat preoptic area-anterior hypothalamus by comparing differentially expressed mRNAs. Adult female rats were ovariectomized and, 1 week later, a silastic capsule containing 17 β-oestradiol (180 μg/ml) was subcutaneously implanted. After 2 days, a single injection of progesterone (1 mg) was administered at 10.00 h and rats were killed at 17.00 h on the same day. Differential-display polymerase chain reaction followed by Northern blot analysis showed that 10 clones were differentially regulated. Using homology search in Genbank, three genes were identified as sodium, potassium-ATPase β1, protein kinase C-binding Nell-homologue protein and evectin-1. Further characterization of 10 clones showed that the expression patterns were tissue-specific and differentially regulated during puberty. Among these, mRNAs for protein kinase C-binding Nell-homologue protein, evectin-1 and human CGI-118 protein-like gene were induced after vagina opening, and differentially expressed during the oestrous cycle. Taken together, several steroid-regulated genes identified in the present study may play an important role in regulating hypothalamic functions, including puberty and the oestrous cycle.
AB - Steroid hormones modulate a variety of physiological functions in the hypothalamus. We attempted to identify steroid-regulated genes in the rat preoptic area-anterior hypothalamus by comparing differentially expressed mRNAs. Adult female rats were ovariectomized and, 1 week later, a silastic capsule containing 17 β-oestradiol (180 μg/ml) was subcutaneously implanted. After 2 days, a single injection of progesterone (1 mg) was administered at 10.00 h and rats were killed at 17.00 h on the same day. Differential-display polymerase chain reaction followed by Northern blot analysis showed that 10 clones were differentially regulated. Using homology search in Genbank, three genes were identified as sodium, potassium-ATPase β1, protein kinase C-binding Nell-homologue protein and evectin-1. Further characterization of 10 clones showed that the expression patterns were tissue-specific and differentially regulated during puberty. Among these, mRNAs for protein kinase C-binding Nell-homologue protein, evectin-1 and human CGI-118 protein-like gene were induced after vagina opening, and differentially expressed during the oestrous cycle. Taken together, several steroid-regulated genes identified in the present study may play an important role in regulating hypothalamic functions, including puberty and the oestrous cycle.
KW - DD-PCR
KW - Genes
KW - Oestrous cycle
KW - POA-AH
KW - Puberty
KW - Steroids
UR - http://www.scopus.com/inward/record.url?scp=0034984398&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034984398&partnerID=8YFLogxK
U2 - 10.1046/j.1365-2826.2001.00665.x
DO - 10.1046/j.1365-2826.2001.00665.x
M3 - Article
VL - 13
SP - 531
EP - 539
JO - Journal of Neuroendocrinology
JF - Journal of Neuroendocrinology
SN - 0953-8194
IS - 6
ER -