Analysis of the cell lysate proteome of a Korean Mycobacterium tuberculosis isolate K01 with H37Rv and H37Ra strains

Sung Weon Ryu, Sang Chan Park, Mun Nam Bang, Sung Sik Han, Young Kil Park, Sue Nie Park, Young Soo Shim, Seong Man Kang, Gill Han Bai

Research output: Contribution to journalArticle

Abstract

Despite recent economic prosperity, Korea still has high prevalence of tuberculosis. Molecular biologic characterization of Korean Mycobacterium tuberculosis strains might provide a deeper understanding of the forces contributing to the spread of tuberculosis in Korea. Therefore, we analyzed the cell lysate proteome of a representative Korean Mycobacterium tuberculosis isolate (K01) in comparison with laboratory reference strains H37Rv and H37Ra. Seven spots were strongly expressed only in K01 strain compared with M. tuberculosis H37Rv and H37Ra. Through continuous MALDI-MS analysis, these spots were identified as hypothetical protein Rv3849, secreted immunogenic protein Mpt64, Acetyl/propionyl-CoA Carbpxylase (AccD1), alkyl hydroperoxide reductase C (AhpC), N-acetylmuramyl-L-alanine amidase, a putative UDP glucose epimerase, and a transposase. A deeper study of these proteins may provide a clue in the development of effective new anti-tuberculosis vaccines against Korean M. tuberculosis isolates.

Original languageEnglish
Pages (from-to)107-112
Number of pages6
JournalJournal of Bacteriology and Virology
Volume34
Issue number2
Publication statusPublished - 2004 Dec 1

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Proteome
Mycobacterium tuberculosis
Korea
amidase
Tuberculosis
UDPglucose 4-Epimerase
Tuberculosis Vaccines
Transposases
Peroxiredoxins
Acetyl Coenzyme A
Proteins
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Alanine
Economics

Keywords

  • 2-D electrophoresis
  • Cytosol protein
  • H37Ra
  • K01
  • M. Tuberculosis H37RV
  • MALDI-MS
  • Proteome

ASJC Scopus subject areas

  • Microbiology
  • Virology
  • Immunology

Cite this

Analysis of the cell lysate proteome of a Korean Mycobacterium tuberculosis isolate K01 with H37Rv and H37Ra strains. / Ryu, Sung Weon; Park, Sang Chan; Bang, Mun Nam; Han, Sung Sik; Park, Young Kil; Park, Sue Nie; Shim, Young Soo; Kang, Seong Man; Bai, Gill Han.

In: Journal of Bacteriology and Virology, Vol. 34, No. 2, 01.12.2004, p. 107-112.

Research output: Contribution to journalArticle

Ryu, Sung Weon ; Park, Sang Chan ; Bang, Mun Nam ; Han, Sung Sik ; Park, Young Kil ; Park, Sue Nie ; Shim, Young Soo ; Kang, Seong Man ; Bai, Gill Han. / Analysis of the cell lysate proteome of a Korean Mycobacterium tuberculosis isolate K01 with H37Rv and H37Ra strains. In: Journal of Bacteriology and Virology. 2004 ; Vol. 34, No. 2. pp. 107-112.
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