Analysis of the effects diclofenac has on Japanese medaka (Oryzias latipes) using real-time PCR

Han N. Hong, Han N. Kim, Kyeong Seo Park, Sung K. Lee, Man Bock Gu

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89 Citations (Scopus)

Abstract

The expression levels of cytochrome P450 1A, p53 and vitellogenin were investigated in three different tissues of male medaka fish after exposure to diclofenac that is one of the main concerns among pharmaceuticals frequently found in sewage treatment plant (STP) effluents. The results showed that cytochrome P450 1A, p53 and vitellogenin were highly expressed in tissue-specific gene expression patterns after exposure to 8 mg/l and 1 μg/l of diclofenac. These elevated expression levels of three biomarkers suggested that diclofenac has potential to cause cellular toxicity, p53-related genotoxicity and estrogenic effects. It is also noteworthy that diclofenac has the potential to cause these effects even at an environmentally relevant concentration of diclofenac, 1 μg/l.

Original languageEnglish
Pages (from-to)2115-2121
Number of pages7
JournalChemosphere
Volume67
Issue number11
DOIs
Publication statusPublished - 2007 May 1

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Keywords

  • Diclofenac
  • Japanese medaka
  • Pharmaceuticals
  • Real-time PCR

ASJC Scopus subject areas

  • Environmental Science(all)
  • Environmental Chemistry

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