Abstract
Cytokines are a protein family of regulatory factors derived from tumors and their environmental components that contribute to the growth, invasion and metastasis of breast cancer. However, the way in which tumor progression and cytokines regulate each other is not well understood. In this study, we used an oligoDNA microarray to assess the kinetic expression profile of cytokine genes in tumor tissues and lymph nodes during the progression of tumor growth in mice that had been subcutaneously challenged with breast adenocarcinoma SB5b cells. Our results demonstrated that IL-15, IL-17, IL-18 and IL-18 binding protein (IL-18bp), which are associated with inflammation, were increased in tumor tissues. Conversely, chemokines and their receptors, including CXCR4/CXCL12, CCR7/CCL21, CCL9, CXCL9 and CCL12, were overexpressed in lymph nodes during tumor growth. Furthermore, RT-PCR and Western blot analysis revealed that IL-18, a pro-angiogenic factor in tumors, was up-regulated in tumor tissues. Interestingly, CCR3, IL-1R2, SOCS and IL-20 were up-regulated in tumor tissues, but down-regulated in lymph nodes during tumor growth. This result suggests that the expression of cytokines and cytokine-related genes was differentially regulated, which resulted in a beneficial effect for tumor progression.
| Original language | English |
|---|---|
| Pages (from-to) | 1141-1147 |
| Number of pages | 7 |
| Journal | Oncology Reports |
| Volume | 22 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 2009 Nov 18 |
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Keywords
- Breast cancer
- Cytokine
- DNA microarray
- Interleukin-18
ASJC Scopus subject areas
- Cancer Research
- Oncology
Cite this
Analysis of the expression profiles of cytokines and cytokine-related genes during the progression of breast cancer growth in mice. / Jung, Mi Young; Kim, Seung Hyun; Cho, Dae Ho; Kim, Tae Sung.
In: Oncology Reports, Vol. 22, No. 5, 18.11.2009, p. 1141-1147.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Analysis of the expression profiles of cytokines and cytokine-related genes during the progression of breast cancer growth in mice
AU - Jung, Mi Young
AU - Kim, Seung Hyun
AU - Cho, Dae Ho
AU - Kim, Tae Sung
PY - 2009/11/18
Y1 - 2009/11/18
N2 - Cytokines are a protein family of regulatory factors derived from tumors and their environmental components that contribute to the growth, invasion and metastasis of breast cancer. However, the way in which tumor progression and cytokines regulate each other is not well understood. In this study, we used an oligoDNA microarray to assess the kinetic expression profile of cytokine genes in tumor tissues and lymph nodes during the progression of tumor growth in mice that had been subcutaneously challenged with breast adenocarcinoma SB5b cells. Our results demonstrated that IL-15, IL-17, IL-18 and IL-18 binding protein (IL-18bp), which are associated with inflammation, were increased in tumor tissues. Conversely, chemokines and their receptors, including CXCR4/CXCL12, CCR7/CCL21, CCL9, CXCL9 and CCL12, were overexpressed in lymph nodes during tumor growth. Furthermore, RT-PCR and Western blot analysis revealed that IL-18, a pro-angiogenic factor in tumors, was up-regulated in tumor tissues. Interestingly, CCR3, IL-1R2, SOCS and IL-20 were up-regulated in tumor tissues, but down-regulated in lymph nodes during tumor growth. This result suggests that the expression of cytokines and cytokine-related genes was differentially regulated, which resulted in a beneficial effect for tumor progression.
AB - Cytokines are a protein family of regulatory factors derived from tumors and their environmental components that contribute to the growth, invasion and metastasis of breast cancer. However, the way in which tumor progression and cytokines regulate each other is not well understood. In this study, we used an oligoDNA microarray to assess the kinetic expression profile of cytokine genes in tumor tissues and lymph nodes during the progression of tumor growth in mice that had been subcutaneously challenged with breast adenocarcinoma SB5b cells. Our results demonstrated that IL-15, IL-17, IL-18 and IL-18 binding protein (IL-18bp), which are associated with inflammation, were increased in tumor tissues. Conversely, chemokines and their receptors, including CXCR4/CXCL12, CCR7/CCL21, CCL9, CXCL9 and CCL12, were overexpressed in lymph nodes during tumor growth. Furthermore, RT-PCR and Western blot analysis revealed that IL-18, a pro-angiogenic factor in tumors, was up-regulated in tumor tissues. Interestingly, CCR3, IL-1R2, SOCS and IL-20 were up-regulated in tumor tissues, but down-regulated in lymph nodes during tumor growth. This result suggests that the expression of cytokines and cytokine-related genes was differentially regulated, which resulted in a beneficial effect for tumor progression.
KW - Breast cancer
KW - Cytokine
KW - DNA microarray
KW - Interleukin-18
UR - http://www.scopus.com/inward/record.url?scp=70449397582&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=70449397582&partnerID=8YFLogxK
U2 - 10.3892/or_00000547
DO - 10.3892/or_00000547
M3 - Article
C2 - 19787232
AN - SCOPUS:70449397582
VL - 22
SP - 1141
EP - 1147
JO - Oncology Reports
JF - Oncology Reports
SN - 1021-335X
IS - 5
ER -