Analysis of the immune-inducible genes of Plutella xylostella using expressed sequence tags and cDNA microarray

Jai Hoon Eum, Young Rok Seo, Sung Moon Yoe, Seok Woo Kang, Sung Sik Han

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

In the present study, the complex gene expression responses of Plutella xylostella to microbial challenges and injury were surveyed using a newly constructed expressed sequence tag (EST) clone collection and cDNA microarray analysis. A total of 1132 P. xylostella ESTs were cloned, annotated and categorized by their putative functions; these included proteases, protease inhibitors, recognition molecules and anti-microbial peptides. GeneOntology revealed that 4% of the P. xylostella ESTs corresponded to immunity-related genes potentially involved in innate immunity. We then used microarray analysis to identify 44 genes that were differentially expressed with at least a two-fold expression difference in P. xylostella before and after pathogen challenge. Together, our EST categorization and microarray profiling analyses allowed us to identify 70 genes that should be considered candidate immune response genes, providing important new insights into the molecular events that occur during the innate immune response in P. xylostella.

Original languageEnglish
Pages (from-to)1107-1120
Number of pages14
JournalDevelopmental and Comparative Immunology
Volume31
Issue number11
DOIs
Publication statusPublished - 2007

Keywords

  • Expressed sequence tags (ESTs)
  • Immune response
  • Microarray
  • Plutella xylostella
  • pxCecropin E (pxCecE)
  • pxS-adenosyl-l-homocysteine hydrolase (pxAhh)

ASJC Scopus subject areas

  • Immunology
  • Developmental Biology

Fingerprint Dive into the research topics of 'Analysis of the immune-inducible genes of Plutella xylostella using expressed sequence tags and cDNA microarray'. Together they form a unique fingerprint.

  • Cite this