Analysis of the major histocompatibility complex class I antigen presentation machinery in human lung cancer

Hyunpyo Kim, Mirim Jin, Ick Young Kim, Byung Yoon Ahn, Seongman Kang, Euiju Choi, Joon Kim, Ik Hwan Kim, Kwangseog Ahn

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Tumor cells may alter the expression of proteins involved in antigen processing and presentation, allowing them to avoid recognition and elimination by cytotoxic T cells. In order to investigate whether the major histocompatibility complex (MHC) class I-mediated antigen processing machinery is preserved in human lung cancer cell lines, we examined the expression of multiple components of the MHC class I antigen processing pathway, including transporter associated with antigen processing (TAP), β2-microglobulin, MHC class I molecules, and chaperones which have not been previously examined in this context. Flow cytometry analysis showed that the cell surface expression of MHC class I molecules was downregulated in all of the cell lines. While some cell lines showed no detectable expression of MHC class I molecules, pulse-chase experiments showed that MHC class I molecules were synthesized in the other cell lines but not transported from the endoplasmic reticulum to the cell surface. Low or nondetectable levels of TAP1 and/or TAP2 expression were demonstrated by Western blot analysis in all of the cell lines, representing a variety of lung tissue types. In some cases, this was accompanied by loss of tapasin expression. Our findings suggest that downregulation of antigen processing may be one of the strategies used by tumors to escape immune surveillance. This study provides further information for designing the potential therapeutic applications such as immunotherapy and gene therapy against cancers.

Original languageEnglish
Pages (from-to)346-351
Number of pages6
JournalJournal of microbiology and biotechnology
Volume9
Issue number3
Publication statusPublished - 1999 Jun

Keywords

  • Antigen presentation
  • Cancer
  • Chaperone
  • Immune escape
  • MHC

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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