Analysis of the production of L929 cell-based cytokine, M-CSF, using 3D spheroid culture system

Yong Hun Jung, Kyuhwan Na, Jeahoon Kim, Donghee Choi, Hyunho Kim, Youngkyu Cho, Seok Chung

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A mouse fibroblast cell line, L929, produces the Macrophage colony-stimulating factor(M-csf) which is a hematopoietic growth factor that plays a critical role in the survival and differentiation of hematopoietic cell lineage.[1] In order to supply sufficient M-csf, three conventional methods are available: co-culturing fibroblast, purchasing recombinant M-csf, and applying M-csf conditioned media. However, conventional methods based on 2-D possess severe drawbacks in space limitation, long term culture, and inconsistent M-csf production. To overcome such inefficiency, we introduce simple spheroid based M-csf harvesting method. Comparing with 2-D data, spheroids numbered at 361 ensure space control, long term culture and M-csf production.

Original languageEnglish
Title of host publication21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
PublisherChemical and Biological Microsystems Society
Pages999-1000
Number of pages2
ISBN (Electronic)9780692941836
Publication statusPublished - 2020 Jan 1
Event21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 - Savannah, United States
Duration: 2017 Oct 222017 Oct 26

Publication series

Name21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017

Conference

Conference21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
CountryUnited States
CitySavannah
Period17/10/2217/10/26

Keywords

  • Efficient
  • Fibroblast
  • Macrophage Colony-Stimulating Factor
  • Production
  • Spheroid

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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  • Cite this

    Jung, Y. H., Na, K., Kim, J., Choi, D., Kim, H., Cho, Y., & Chung, S. (2020). Analysis of the production of L929 cell-based cytokine, M-CSF, using 3D spheroid culture system. In 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 (pp. 999-1000). (21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017). Chemical and Biological Microsystems Society.