Analysis of the stability of HLA-A2 molecules expressed on the cell surface

Jong Seok Lim, Ki Young Lee, Hee Gu Lee, Ik Hwan Kim, Chong Kil Lee, Seong Sun Han, Kilhyoun Kim

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Association of antigenic peptide with class I MHC is believed to be crucial for maintaining stable conformation of class I molecules. T2 cells that are defective in TAP gene function mainly express class I molecules with an unstable conformation due to little or no association with antigenic peptides, whereas T1 cells that are normal in TAP gene function mainly express the stable form of class I molecules. In this work, attempts were made to determine the molecular stability of stable and unstable class I molecules. Dissociation of HLA-A2 molecules on T1 and T2 cells was monitored by flow cytomerry using anti-HLA-A2 antibody after the cells were treated with brefeldin A to shut down the transport of newly-assembled HLA-A2. Estimated dissociation rate constants for the stable and unstable forms of HLA-A2 were 0.076 h-1 and 0.66 h-1, respectively. It appeared that both T1 and T2 cells express stable and unstable class I complex, but with different ratios of the two forms. Furthermore, interferon-γ treatment of T1 cells appeared to induce the expression of both the stable and unstable class I molecules. These results demonstrate that class I MHC molecules can be divided into two groups in terms of structural stability and that they exist on the cell surface in both forms in a certain ratio.

Original languageEnglish
Pages (from-to)286-293
Number of pages8
JournalJournal of Biochemistry and Molecular Biology
Volume29
Issue number4
Publication statusPublished - 1996 Jul 31
Externally publishedYes

Fingerprint

HLA-A2 Antigen
Molecules
Conformations
Genes
Brefeldin A
Peptides
Interferons
Rate constants
Association reactions
Antibodies

Keywords

  • Cell surface expression
  • Dissociation rate constant
  • HLA-A2 molecule
  • Interferon-γ

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Lim, J. S., Lee, K. Y., Lee, H. G., Kim, I. H., Lee, C. K., Han, S. S., & Kim, K. (1996). Analysis of the stability of HLA-A2 molecules expressed on the cell surface. Journal of Biochemistry and Molecular Biology, 29(4), 286-293.

Analysis of the stability of HLA-A2 molecules expressed on the cell surface. / Lim, Jong Seok; Lee, Ki Young; Lee, Hee Gu; Kim, Ik Hwan; Lee, Chong Kil; Han, Seong Sun; Kim, Kilhyoun.

In: Journal of Biochemistry and Molecular Biology, Vol. 29, No. 4, 31.07.1996, p. 286-293.

Research output: Contribution to journalArticle

Lim, JS, Lee, KY, Lee, HG, Kim, IH, Lee, CK, Han, SS & Kim, K 1996, 'Analysis of the stability of HLA-A2 molecules expressed on the cell surface', Journal of Biochemistry and Molecular Biology, vol. 29, no. 4, pp. 286-293.
Lim, Jong Seok ; Lee, Ki Young ; Lee, Hee Gu ; Kim, Ik Hwan ; Lee, Chong Kil ; Han, Seong Sun ; Kim, Kilhyoun. / Analysis of the stability of HLA-A2 molecules expressed on the cell surface. In: Journal of Biochemistry and Molecular Biology. 1996 ; Vol. 29, No. 4. pp. 286-293.
@article{affab46ebd024de191dca99661b2d5f4,
title = "Analysis of the stability of HLA-A2 molecules expressed on the cell surface",
abstract = "Association of antigenic peptide with class I MHC is believed to be crucial for maintaining stable conformation of class I molecules. T2 cells that are defective in TAP gene function mainly express class I molecules with an unstable conformation due to little or no association with antigenic peptides, whereas T1 cells that are normal in TAP gene function mainly express the stable form of class I molecules. In this work, attempts were made to determine the molecular stability of stable and unstable class I molecules. Dissociation of HLA-A2 molecules on T1 and T2 cells was monitored by flow cytomerry using anti-HLA-A2 antibody after the cells were treated with brefeldin A to shut down the transport of newly-assembled HLA-A2. Estimated dissociation rate constants for the stable and unstable forms of HLA-A2 were 0.076 h-1 and 0.66 h-1, respectively. It appeared that both T1 and T2 cells express stable and unstable class I complex, but with different ratios of the two forms. Furthermore, interferon-γ treatment of T1 cells appeared to induce the expression of both the stable and unstable class I molecules. These results demonstrate that class I MHC molecules can be divided into two groups in terms of structural stability and that they exist on the cell surface in both forms in a certain ratio.",
keywords = "Cell surface expression, Dissociation rate constant, HLA-A2 molecule, Interferon-γ",
author = "Lim, {Jong Seok} and Lee, {Ki Young} and Lee, {Hee Gu} and Kim, {Ik Hwan} and Lee, {Chong Kil} and Han, {Seong Sun} and Kilhyoun Kim",
year = "1996",
month = "7",
day = "31",
language = "English",
volume = "29",
pages = "286--293",
journal = "BMB Reports",
issn = "1976-6696",
publisher = "The Biochemical Society of the Republic of Korea",
number = "4",

}

TY - JOUR

T1 - Analysis of the stability of HLA-A2 molecules expressed on the cell surface

AU - Lim, Jong Seok

AU - Lee, Ki Young

AU - Lee, Hee Gu

AU - Kim, Ik Hwan

AU - Lee, Chong Kil

AU - Han, Seong Sun

AU - Kim, Kilhyoun

PY - 1996/7/31

Y1 - 1996/7/31

N2 - Association of antigenic peptide with class I MHC is believed to be crucial for maintaining stable conformation of class I molecules. T2 cells that are defective in TAP gene function mainly express class I molecules with an unstable conformation due to little or no association with antigenic peptides, whereas T1 cells that are normal in TAP gene function mainly express the stable form of class I molecules. In this work, attempts were made to determine the molecular stability of stable and unstable class I molecules. Dissociation of HLA-A2 molecules on T1 and T2 cells was monitored by flow cytomerry using anti-HLA-A2 antibody after the cells were treated with brefeldin A to shut down the transport of newly-assembled HLA-A2. Estimated dissociation rate constants for the stable and unstable forms of HLA-A2 were 0.076 h-1 and 0.66 h-1, respectively. It appeared that both T1 and T2 cells express stable and unstable class I complex, but with different ratios of the two forms. Furthermore, interferon-γ treatment of T1 cells appeared to induce the expression of both the stable and unstable class I molecules. These results demonstrate that class I MHC molecules can be divided into two groups in terms of structural stability and that they exist on the cell surface in both forms in a certain ratio.

AB - Association of antigenic peptide with class I MHC is believed to be crucial for maintaining stable conformation of class I molecules. T2 cells that are defective in TAP gene function mainly express class I molecules with an unstable conformation due to little or no association with antigenic peptides, whereas T1 cells that are normal in TAP gene function mainly express the stable form of class I molecules. In this work, attempts were made to determine the molecular stability of stable and unstable class I molecules. Dissociation of HLA-A2 molecules on T1 and T2 cells was monitored by flow cytomerry using anti-HLA-A2 antibody after the cells were treated with brefeldin A to shut down the transport of newly-assembled HLA-A2. Estimated dissociation rate constants for the stable and unstable forms of HLA-A2 were 0.076 h-1 and 0.66 h-1, respectively. It appeared that both T1 and T2 cells express stable and unstable class I complex, but with different ratios of the two forms. Furthermore, interferon-γ treatment of T1 cells appeared to induce the expression of both the stable and unstable class I molecules. These results demonstrate that class I MHC molecules can be divided into two groups in terms of structural stability and that they exist on the cell surface in both forms in a certain ratio.

KW - Cell surface expression

KW - Dissociation rate constant

KW - HLA-A2 molecule

KW - Interferon-γ

UR - http://www.scopus.com/inward/record.url?scp=0030519998&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030519998&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0030519998

VL - 29

SP - 286

EP - 293

JO - BMB Reports

JF - BMB Reports

SN - 1976-6696

IS - 4

ER -