Analysis of the stress effects of endocrine disrupting chemicals (EDCs) on Escherichia coli

Yeon Seok Kim, Jiho Min, Han Na Hong, Ji Hyun Park, Kyeong Seo Park, Man Bock Gu

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

In this study, three of the representative EDCs, 17β-estradiol, bisphenol A, and styrene, were employed to find their mode of toxic actions in E. coli. To accomplish this, four different stress response genes, recA, katG, fabA, and grpE genes, were used as a representative for DNA, oxidative, membrane, or protein damage, respectively. The expression levels of these four genes were quantified using a real-time RT-PCR after challenge with three different EDCs individually. Bisphenol A and styrene caused high-level expression of recA and katG genes, respectively, whereas 17β-estradiol made no significant changes in expression of any of those genes. These results lead to the classification of the mode of toxic actions of EDCs on E. coli.

Original languageEnglish
Pages (from-to)1390-1393
Number of pages4
JournalJournal of Microbiology and Biotechnology
Volume17
Issue number8
Publication statusPublished - 2007 Aug 1

Fingerprint

Endocrine Disruptors
Escherichia coli
Genes
Toxic Actions
Styrene
Estradiol
Real-Time Polymerase Chain Reaction
Membrane Proteins
DNA
Proteins
Membranes

Keywords

  • Endocrine disrupting chemicals (EDCs)
  • Gene expression analysis
  • Real-time RT-PCR
  • Stress effect
  • Stress-specific responsive gene

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Microbiology
  • Bioengineering

Cite this

Analysis of the stress effects of endocrine disrupting chemicals (EDCs) on Escherichia coli. / Kim, Yeon Seok; Min, Jiho; Hong, Han Na; Park, Ji Hyun; Park, Kyeong Seo; Gu, Man Bock.

In: Journal of Microbiology and Biotechnology, Vol. 17, No. 8, 01.08.2007, p. 1390-1393.

Research output: Contribution to journalArticle

Kim, Yeon Seok ; Min, Jiho ; Hong, Han Na ; Park, Ji Hyun ; Park, Kyeong Seo ; Gu, Man Bock. / Analysis of the stress effects of endocrine disrupting chemicals (EDCs) on Escherichia coli. In: Journal of Microbiology and Biotechnology. 2007 ; Vol. 17, No. 8. pp. 1390-1393.
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