Analysis of the toxic mode of action of silver nanoparticles using stress-specific bioluminescent bacteria

Ee Taek Hwang, Jin Hyung Lee, Yun Ju Chae, Yeon Seok Kim, Byoung Chan Kim, Byoung In Sang, Man Bock Gu

Research output: Contribution to journalArticle

265 Citations (Scopus)

Abstract

A study was conducted to investigate the effect of toxic mode of silver nanoparticles on cell membrane using stress-specific biloluminescent bacteria. The cytotoxicity of silver nanoparticles in growth inhibition was tested with the wild-type E.coli strain, RFM443. Scientist used high-angle annular dark-field scanning transmission electron microscopy (HAADF-STEM) to identify the effect of silver nanoparticles on bacteria. It was founnd that silver nanoparticles attached to the surface of the cell membrane and penerated inside the cell. A silver nanoparticles generated Ag+ ions, which were partially responsible for the biocidal effect. Scientist also evaluated the cell viability and other effects caused by the silver nanoparticles were analyzed using scanning electron microscopy (SEM). It is concluded that silver nanoparticles cause toxicity through protein/membrane and oxidative damage, but do not result in DNA damage.

Original languageEnglish
Pages (from-to)746-750
Number of pages5
JournalSmall
Volume4
Issue number6
DOIs
Publication statusPublished - 2008 Jun 1

Fingerprint

Toxic Actions
Poisons
Silver
Nanoparticles
Bacteria
Cell membranes
Cell Membrane
Scanning Transmission Electron Microscopy
Scanning electron microscopy
Cytotoxicity
Electron Scanning Microscopy
Escherichia coli
DNA Damage
Toxicity
Cell Survival
Membrane Proteins
DNA
Cells
Ions
Transmission electron microscopy

Keywords

  • Bioluminescence
  • Cell damage
  • Reactive oxygen species
  • Silver nanoparticles
  • Toxicology

ASJC Scopus subject areas

  • Biomaterials
  • Engineering (miscellaneous)
  • Biotechnology
  • Medicine(all)

Cite this

Analysis of the toxic mode of action of silver nanoparticles using stress-specific bioluminescent bacteria. / Hwang, Ee Taek; Lee, Jin Hyung; Chae, Yun Ju; Kim, Yeon Seok; Kim, Byoung Chan; Sang, Byoung In; Gu, Man Bock.

In: Small, Vol. 4, No. 6, 01.06.2008, p. 746-750.

Research output: Contribution to journalArticle

Hwang, Ee Taek ; Lee, Jin Hyung ; Chae, Yun Ju ; Kim, Yeon Seok ; Kim, Byoung Chan ; Sang, Byoung In ; Gu, Man Bock. / Analysis of the toxic mode of action of silver nanoparticles using stress-specific bioluminescent bacteria. In: Small. 2008 ; Vol. 4, No. 6. pp. 746-750.
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