Analysis of zinc oxide nanoparticles binding proteins in rat blood and brain homogenate

Kyu Hwan Shim, John Hulme, Eun Ho Maeng, Meyoung-Kon Kim, Seong Soo A. An

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Nanoparticles (NPs) are currently used in chemical, cosmetic, pharmaceutical, and electronic products. Nevertheless, limited safety information is available for many NPs, especially in terms of their interactions with various binding proteins, leading to potential toxic effects. Zinc oxide (ZnO) NPs are included in the formulation of new products, such as adhesives, batteries, ceramics, cosmetics, cement, glass, ointments, paints, pigments, and supplementary foods, resulting in increased human exposures to ZnO. Hence, we investigated the potential ZnO nanotoxic pathways by analyzing the adsorbed proteins, called protein corona, from blood and brain from four ZnO NPs, ZnOSM20(−), ZnOSM20(+), ZnOAE100(−), and ZnOAE100(+), in order to understand their potential mechanisms in vivo. Through this study, liquid chromatography–mass spectroscopy/ mass spectroscopy technology was employed to identify all bound proteins. Totals of 52 and 58 plasma proteins were identified as being bound to ZnOSM20(−) and ZnOSM20(+), respectively. For ZnOAE100(−) and ZnOAE100(+), 58 and 44 proteins were bound, respectively. Similar numbers of proteins were adsorbed onto ZnO irrespective of size or surface charge of the nanoparticle. These proteins were further analyzed with ClueGO, a Cytoscape plugin, which provided gene ontology and the biological interaction processes of identified proteins. Interactions between diverse proteins and ZnO nanoparticles could result in an alteration of their functions, conformation, and clearance, eventually affecting many biological processes.

Original languageEnglish
Pages (from-to)217-224
Number of pages8
JournalInternational Journal of Nanomedicine
Volume9
DOIs
Publication statusPublished - 2014 Dec 15

Fingerprint

Zinc Oxide
Zinc oxide
Nanoparticles
Rats
Brain
Carrier Proteins
Blood
Proteins
Biological Phenomena
Cosmetics
Spectroscopy
Gene Ontology
Paint
Poisons
Ceramics
Surface charge
Ointments
Pigments
Adhesives
Glass

Keywords

  • Brain homogenate
  • Nanotoxicity
  • Plasma
  • Protein corona

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Drug Discovery
  • Organic Chemistry

Cite this

Analysis of zinc oxide nanoparticles binding proteins in rat blood and brain homogenate. / Shim, Kyu Hwan; Hulme, John; Maeng, Eun Ho; Kim, Meyoung-Kon; An, Seong Soo A.

In: International Journal of Nanomedicine, Vol. 9, 15.12.2014, p. 217-224.

Research output: Contribution to journalArticle

Shim, Kyu Hwan ; Hulme, John ; Maeng, Eun Ho ; Kim, Meyoung-Kon ; An, Seong Soo A. / Analysis of zinc oxide nanoparticles binding proteins in rat blood and brain homogenate. In: International Journal of Nanomedicine. 2014 ; Vol. 9. pp. 217-224.
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