Angiogenic activity of human CC chemokine CCL15 in vitro and in vivo

Jungsu Hwang, Chan Woo Kim, Kyung No Son, Kyu Yeon Han, Kyung Hee Lee, Hynda K. Kleinman, Jesang Ko, Doe Sun Na, Byoung S. Kwon, Yong Song Gho, Jiyoung Kim

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

CCL15 is a novel human CC chemokine and exerts its biological activities on immune cells through CCR1 and CCR3. Because a number of chemokines induce angiogenesis and endothelial cells express CCR1 and CCR3, we investigated the angiogenic activity of CCL15. Both CCL15(1-92) and N-terminal truncated CCL15(25-92) stimulate the chemotactic endothelial cell migration and differentiation, but CCL15(25-92) is at least 100-fold more potent than CCL15(1-92). Treatment with pertussis toxin (PTX), with anti-CCR1, or with anti-CCR3 antibody inhibits the CCL15(25-92)-induced endothelial cell migration. CCL15(25-92) also stimulates sprouting of vessels from aortic rings and mediates angiogenesis in the chick chorioallantoic membrane assay. Our findings demonstrate that CCL15(25-92) has in vitro and in vivo angiogenic activity, and suggest roles of the chemokine in angiogenesis.

Original languageEnglish
Pages (from-to)47-51
Number of pages5
JournalFEBS Letters
Volume570
Issue number1-3
DOIs
Publication statusPublished - 2004 Jul 16

Keywords

  • Angiogenesis
  • CCL15
  • CCR1
  • CCR3
  • Chemokine
  • Endothelial cells

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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  • Cite this

    Hwang, J., Kim, C. W., Son, K. N., Han, K. Y., Lee, K. H., Kleinman, H. K., Ko, J., Na, D. S., Kwon, B. S., Gho, Y. S., & Kim, J. (2004). Angiogenic activity of human CC chemokine CCL15 in vitro and in vivo. FEBS Letters, 570(1-3), 47-51. https://doi.org/10.1016/j.febslet.2004.06.023