Angiogenic activity of human CC chemokine CCL15 in vitro and in vivo

Jungsu Hwang, Chan Woo Kim, Kyung No Son, Kyu Yeon Han, Kyung Hee Lee, Hynda K. Kleinman, Je Sang Ko, Doe Sun Na, Byoung S. Kwon, Yong Song Gho, Jiyoung Kim

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

CCL15 is a novel human CC chemokine and exerts its biological activities on immune cells through CCR1 and CCR3. Because a number of chemokines induce angiogenesis and endothelial cells express CCR1 and CCR3, we investigated the angiogenic activity of CCL15. Both CCL15(1-92) and N-terminal truncated CCL15(25-92) stimulate the chemotactic endothelial cell migration and differentiation, but CCL15(25-92) is at least 100-fold more potent than CCL15(1-92). Treatment with pertussis toxin (PTX), with anti-CCR1, or with anti-CCR3 antibody inhibits the CCL15(25-92)-induced endothelial cell migration. CCL15(25-92) also stimulates sprouting of vessels from aortic rings and mediates angiogenesis in the chick chorioallantoic membrane assay. Our findings demonstrate that CCL15(25-92) has in vitro and in vivo angiogenic activity, and suggest roles of the chemokine in angiogenesis.

Original languageEnglish
Pages (from-to)47-51
Number of pages5
JournalFEBS Letters
Volume570
Issue number1-3
DOIs
Publication statusPublished - 2004 Jul 16
Externally publishedYes

Fingerprint

CC Chemokines
Endothelial cells
Human Activities
Endothelial Cells
Chemokines
Cell Movement
Chorioallantoic Membrane
Pertussis Toxin
Bioactivity
Cell Differentiation
Anti-Idiotypic Antibodies
Assays
Membranes
Antibodies
In Vitro Techniques

Keywords

  • Angiogenesis
  • CCL15
  • CCR1
  • CCR3
  • Chemokine
  • Endothelial cells

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Hwang, J., Kim, C. W., Son, K. N., Han, K. Y., Lee, K. H., Kleinman, H. K., ... Kim, J. (2004). Angiogenic activity of human CC chemokine CCL15 in vitro and in vivo. FEBS Letters, 570(1-3), 47-51. https://doi.org/10.1016/j.febslet.2004.06.023

Angiogenic activity of human CC chemokine CCL15 in vitro and in vivo. / Hwang, Jungsu; Kim, Chan Woo; Son, Kyung No; Han, Kyu Yeon; Lee, Kyung Hee; Kleinman, Hynda K.; Ko, Je Sang; Na, Doe Sun; Kwon, Byoung S.; Gho, Yong Song; Kim, Jiyoung.

In: FEBS Letters, Vol. 570, No. 1-3, 16.07.2004, p. 47-51.

Research output: Contribution to journalArticle

Hwang, J, Kim, CW, Son, KN, Han, KY, Lee, KH, Kleinman, HK, Ko, JS, Na, DS, Kwon, BS, Gho, YS & Kim, J 2004, 'Angiogenic activity of human CC chemokine CCL15 in vitro and in vivo', FEBS Letters, vol. 570, no. 1-3, pp. 47-51. https://doi.org/10.1016/j.febslet.2004.06.023
Hwang J, Kim CW, Son KN, Han KY, Lee KH, Kleinman HK et al. Angiogenic activity of human CC chemokine CCL15 in vitro and in vivo. FEBS Letters. 2004 Jul 16;570(1-3):47-51. https://doi.org/10.1016/j.febslet.2004.06.023
Hwang, Jungsu ; Kim, Chan Woo ; Son, Kyung No ; Han, Kyu Yeon ; Lee, Kyung Hee ; Kleinman, Hynda K. ; Ko, Je Sang ; Na, Doe Sun ; Kwon, Byoung S. ; Gho, Yong Song ; Kim, Jiyoung. / Angiogenic activity of human CC chemokine CCL15 in vitro and in vivo. In: FEBS Letters. 2004 ; Vol. 570, No. 1-3. pp. 47-51.
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