Anisotropically organized three-dimensional culture platform for reconstruction of a hippocampal neural network

So Hyun Kim, Sun Kyoung Im, Soo Jin Oh, Sohyeon Jeong, Eui Sung Yoon, Changjoon Lee, Nakwon Choi, Eun Mi Hur

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

In native tissues, cellular and acellular components are anisotropically organized and often aligned in specific directions, providing structural and mechanical properties for actuating biological functions. Thus, engineering alignment not only allows for emulation of native tissue structures but might also enable implementation of specific functionalities. However, achieving desired alignment is challenging, especially in three-dimensional constructs. By exploiting the elastomeric property of polydimethylsiloxane and fibrillogenesis kinetics of collagen, here we introduce a simple yet effective method to assemble and align fibrous structures in a multi-modular three-dimensional conglomerate. Applying this method, we have reconstructed the CA3-CA1 hippocampal neural circuit three-dimensionally in a monolithic gel, in which CA3 neurons extend parallel axons to and synapse with CA1 neurons. Furthermore, we show that alignment of the fibrous scaffold facilitates the establishment of functional connectivity. This method can be applied for reconstructing other neural circuits or tissue units where anisotropic organization in a multi-modular structure is desired.

Original languageEnglish
Article number14346
JournalNature Communications
Volume8
DOIs
Publication statusPublished - 2017 Feb 1

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platforms
alignment
Tissue
Neural networks
neurons
Neurons
axons
synapses
Networks (circuits)
collagens
Scaffolds
Synapses
Axons
Structural properties
Collagen
Gels
engineering
gels
mechanical properties
Mechanical properties

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Anisotropically organized three-dimensional culture platform for reconstruction of a hippocampal neural network. / Kim, So Hyun; Im, Sun Kyoung; Oh, Soo Jin; Jeong, Sohyeon; Yoon, Eui Sung; Lee, Changjoon; Choi, Nakwon; Hur, Eun Mi.

In: Nature Communications, Vol. 8, 14346, 01.02.2017.

Research output: Contribution to journalArticle

Kim, So Hyun ; Im, Sun Kyoung ; Oh, Soo Jin ; Jeong, Sohyeon ; Yoon, Eui Sung ; Lee, Changjoon ; Choi, Nakwon ; Hur, Eun Mi. / Anisotropically organized three-dimensional culture platform for reconstruction of a hippocampal neural network. In: Nature Communications. 2017 ; Vol. 8.
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