Abstract
Both in vivo and in vitro, specific sequences in double-stranded DNA can adopt the left-handed Z-form when underwound. Recently, the B-Z transition of DNA has been studied in detail in magnetic tweezers experiments by several groups. We present a theoretical description of this transition, based on an annealed random copolymer model. The transition of a switchable sequence is discussed as a function of energetic and geometric parameters of the B- and Z-forms, of the applied boundary conditions, and of the characteristics of the B-Z interface. We address a possible torsional softening upon the B-Z transition. The model can be also applied to other biofilaments with annealed torsional/flexural degrees of freedom.
| Original language | English |
|---|---|
| Pages (from-to) | 2562-2572 |
| Number of pages | 11 |
| Journal | Biophysical Journal |
| Volume | 108 |
| Issue number | 10 |
| DOIs | |
| Publication status | Published - 2015 May 19 |
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ASJC Scopus subject areas
- Biophysics
Cite this
Annealed random copolymer model of the B-Z transition in DNA : Torsional responses. / Kwon, Ah Young; Lee, Nam Kyung; Hong, Seok Cheol; Fierling, Julien; Johner, Albert.
In: Biophysical Journal, Vol. 108, No. 10, 19.05.2015, p. 2562-2572.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Annealed random copolymer model of the B-Z transition in DNA
T2 - Torsional responses
AU - Kwon, Ah Young
AU - Lee, Nam Kyung
AU - Hong, Seok Cheol
AU - Fierling, Julien
AU - Johner, Albert
PY - 2015/5/19
Y1 - 2015/5/19
N2 - Both in vivo and in vitro, specific sequences in double-stranded DNA can adopt the left-handed Z-form when underwound. Recently, the B-Z transition of DNA has been studied in detail in magnetic tweezers experiments by several groups. We present a theoretical description of this transition, based on an annealed random copolymer model. The transition of a switchable sequence is discussed as a function of energetic and geometric parameters of the B- and Z-forms, of the applied boundary conditions, and of the characteristics of the B-Z interface. We address a possible torsional softening upon the B-Z transition. The model can be also applied to other biofilaments with annealed torsional/flexural degrees of freedom.
AB - Both in vivo and in vitro, specific sequences in double-stranded DNA can adopt the left-handed Z-form when underwound. Recently, the B-Z transition of DNA has been studied in detail in magnetic tweezers experiments by several groups. We present a theoretical description of this transition, based on an annealed random copolymer model. The transition of a switchable sequence is discussed as a function of energetic and geometric parameters of the B- and Z-forms, of the applied boundary conditions, and of the characteristics of the B-Z interface. We address a possible torsional softening upon the B-Z transition. The model can be also applied to other biofilaments with annealed torsional/flexural degrees of freedom.
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UR - http://www.scopus.com/inward/citedby.url?scp=84929989715&partnerID=8YFLogxK
U2 - 10.1016/j.bpj.2015.03.060
DO - 10.1016/j.bpj.2015.03.060
M3 - Article
C2 - 25992734
AN - SCOPUS:84929989715
VL - 108
SP - 2562
EP - 2572
JO - Biophysical Journal
JF - Biophysical Journal
SN - 0006-3495
IS - 10
ER -