Anoctamin-1 affects the migration and invasion of anaplastic thyroid carcinoma cells

Jae Young Kim; Hwa Young Youn; June Choi; Seung Kuk Baek; Soon Young Kwon; Bok Kee Eun; Jae Yong Park; Kyoung Ho Oh

doi:10.1080/19768354.2019.1614981

Anoctamin-1 affects the migration and invasion of anaplastic thyroid carcinoma cells

Jae Young Kim, Hwa Young Youn, June Choi, Seung Kuk Baek, Soon Young Kwon, Bok Kee Eun, Jae Yong Park, Kyoung Ho Oh

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

Anaplastic thyroid carcinoma (ATC) is a rare malignancy with very poor prognosis. The exact cause underlying its strong aggressive nature is not clear. Here, we discovered the elevated expression of anoctamin-1 (ANO1; Ca²⁺-activated Cl⁻ channels) in advanced-stage ATC tissue. Using different ATC cell lines, the degree of expression of ANO1 was found to be related to the degree of ATC cell invasion by quantitative reverse transcription polymerase chain reaction and western blotting. Suppression of ANO1 activity either by selective inhibitor (T16Ainh-A01) or by siRNA significantly attenuated the migration and invasion of ATC cells. In conclusion, ANO1 appears to increase the ability of ATC cells to invade and migrate. Our results also suggest that the expression of ANO1 in patients with ATC may be helpful in predicting the prognosis of ATC.

Original language	English
Pages (from-to)	294-301
Number of pages	8
Journal	Animal Cells and Systems
Volume	23
Issue number	4
DOIs	https://doi.org/10.1080/19768354.2019.1614981
Publication status	Published - 2019 Jul 4

Keywords

Anoctamin
invasion
migration
thyroid cancer

ASJC Scopus subject areas

Animal Science and Zoology
Biochemistry, Genetics and Molecular Biology(all)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1080/19768354.2019.1614981

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title = "Anoctamin-1 affects the migration and invasion of anaplastic thyroid carcinoma cells",

abstract = "Anaplastic thyroid carcinoma (ATC) is a rare malignancy with very poor prognosis. The exact cause underlying its strong aggressive nature is not clear. Here, we discovered the elevated expression of anoctamin-1 (ANO1; Ca2+-activated Cl− channels) in advanced-stage ATC tissue. Using different ATC cell lines, the degree of expression of ANO1 was found to be related to the degree of ATC cell invasion by quantitative reverse transcription polymerase chain reaction and western blotting. Suppression of ANO1 activity either by selective inhibitor (T16Ainh-A01) or by siRNA significantly attenuated the migration and invasion of ATC cells. In conclusion, ANO1 appears to increase the ability of ATC cells to invade and migrate. Our results also suggest that the expression of ANO1 in patients with ATC may be helpful in predicting the prognosis of ATC.",

keywords = "Anoctamin, invasion, migration, thyroid cancer",

author = "Kim, {Jae Young} and Youn, {Hwa Young} and June Choi and Baek, {Seung Kuk} and Kwon, {Soon Young} and Eun, {Bok Kee} and Park, {Jae Yong} and Oh, {Kyoung Ho}",

note = "Funding Information: This work was supported by Korea University. Publisher Copyright: {\textcopyright} 2019, {\textcopyright} 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.",

year = "2019",

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T1 - Anoctamin-1 affects the migration and invasion of anaplastic thyroid carcinoma cells

AU - Kim, Jae Young

AU - Youn, Hwa Young

AU - Choi, June

AU - Baek, Seung Kuk

AU - Kwon, Soon Young

AU - Eun, Bok Kee

AU - Park, Jae Yong

AU - Oh, Kyoung Ho

PY - 2019/7/4

Y1 - 2019/7/4

N2 - Anaplastic thyroid carcinoma (ATC) is a rare malignancy with very poor prognosis. The exact cause underlying its strong aggressive nature is not clear. Here, we discovered the elevated expression of anoctamin-1 (ANO1; Ca2+-activated Cl− channels) in advanced-stage ATC tissue. Using different ATC cell lines, the degree of expression of ANO1 was found to be related to the degree of ATC cell invasion by quantitative reverse transcription polymerase chain reaction and western blotting. Suppression of ANO1 activity either by selective inhibitor (T16Ainh-A01) or by siRNA significantly attenuated the migration and invasion of ATC cells. In conclusion, ANO1 appears to increase the ability of ATC cells to invade and migrate. Our results also suggest that the expression of ANO1 in patients with ATC may be helpful in predicting the prognosis of ATC.

AB - Anaplastic thyroid carcinoma (ATC) is a rare malignancy with very poor prognosis. The exact cause underlying its strong aggressive nature is not clear. Here, we discovered the elevated expression of anoctamin-1 (ANO1; Ca2+-activated Cl− channels) in advanced-stage ATC tissue. Using different ATC cell lines, the degree of expression of ANO1 was found to be related to the degree of ATC cell invasion by quantitative reverse transcription polymerase chain reaction and western blotting. Suppression of ANO1 activity either by selective inhibitor (T16Ainh-A01) or by siRNA significantly attenuated the migration and invasion of ATC cells. In conclusion, ANO1 appears to increase the ability of ATC cells to invade and migrate. Our results also suggest that the expression of ANO1 in patients with ATC may be helpful in predicting the prognosis of ATC.

KW - Anoctamin

KW - invasion

KW - migration

KW - thyroid cancer

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VL - 23

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ER -

Anoctamin-1 affects the migration and invasion of anaplastic thyroid carcinoma cells

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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