Anteroposterior Wnt-RA Gradient Defines Adhesion and Migration Properties of Neural Progenitors in Developing Spinal Cord

Mohammed R. Shaker, Ju Hyun Lee, Si Hyung Park, Joo Yeon Kim, Gi Hoon Son, Jong Wan Son, Bae Ho Park, Im Joo Rhyu, Hyun Kim, Woong Sun

Research output: Contribution to journalArticlepeer-review

Abstract

Mammalian embryos exhibit a transition from head morphogenesis to trunk elongation to meet the demand of axial elongation. The caudal neural tube (NT) is formed with neural progenitors (NPCs) derived from neuromesodermal progenitors localized at the tail tip. However, the molecular and cellular basis of elongating NT morphogenesis is yet elusive. Here, we provide evidence that caudal NPCs exhibit strong adhesion affinity that is gradually decreased along the anteroposterior (AP) axis in mouse embryonic spinal cord and human cellular models. Strong cell-cell adhesion causes collective migration, allowing AP alignment of NPCs depending on their birthdate. We further validated that this axial adhesion gradient is associated with the extracellular matrix and is under the control of graded Wnt signaling emanating from tail buds and antagonistic retinoic acid (RA) signaling. These results suggest that progressive reduction of NPC adhesion along the AP axis is under the control of Wnt-RA molecular networks, which is essential for a proper elongation of the spinal cord.

Original languageEnglish
Pages (from-to)898-911
Number of pages14
JournalStem Cell Reports
Volume15
Issue number4
DOIs
Publication statusPublished - 2020 Oct 13

Keywords

  • Wnt-RA network
  • adhesion and migration
  • axial elongation
  • extracellular matrix
  • neural stem cells
  • neuromesodermal progenitors

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Developmental Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Anteroposterior Wnt-RA Gradient Defines Adhesion and Migration Properties of Neural Progenitors in Developing Spinal Cord'. Together they form a unique fingerprint.

Cite this